Associations between toll-like receptors and interleukin-4 in the lungs of patients with tuberculosis
Associations between toll-like receptors and interleukin-4 in the lungs of patients with tuberculosis
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DOI：
10.1165/rcmb.2002-0163oc
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发表时间：
2003-07-01
影响因子：
6.4
通讯作者：
Lukey, PT
中科院分区：
文献类型：
--
作者：
Fenhalls, G;Squires, GR;Lukey, PT
toll样受体（TLRs）与结核分枝杆菌的细胞内杀伤有关，其表达在体外受白细胞介素-4 （IL-4）调节。我们的目的是检测结核性肺肉芽肿病理部位TLR的表达，并探讨免疫应答对TLR表达的影响。对9例因咯血而行肺叶切除术的肺结核患者的肺肉芽肿进行免疫组化。所有9例患者均表达了所研究的所有TLRs (TLRs 1-5和9)，而9例患者中只有5例存在IL-4阳性肉芽肿。对9例患者183个肉芽肿的TLR和细胞因子染色模式进行统计分析，发现受体对与IL-4之间存在显著相关性。TLR2与TLR4呈正相关（P < 0.0001）， TLR2与IL-4呈负相关（P < 0.0001）。与il -4阳性患者相比，il -4阴性患者的TLRs 1、5和9的相关性有显著差异。综上所述，tlr在人结核性肺的多种细胞类型中均有表达，其表达模式可通过免疫应答的差异来反映。
Toll-like receptors (TLRs) are implicated in the intracellular killing of Mycobacterium tuberculosis, and their expression is modulated by interieukin-4 (IL-4) in vitro. Our aim was to examine the expression of TLRs at the site of pathology in tuberculous lung granulomas and to explore the effect of the immune response on TLR expression. Immunohistochemistry was performed on lung granulomas from nine patients with tuberculosis undergoing lobectomy for haemoptysis. All nine patients expressed all of the TLRs studied (TLRs 1-5 and 9), whereas only five out of the nine patients had any granulomas positive for IL-4. Statistical analysis of TLR and cytokine staining patterns in 183 individual granulomas from the nine patients revealed significant associations between pairs of receptors and IL-4. A positive association between TLR2 and TLR4 (P < 0.0001) and a negative association between TLR2 and IL-4 (P < 0.0001) was observed. The associations between TLRs 1, 5, and 9 were significantly different in IL-4-negative compared with IL-4-positive patients. In conclusion, TLRs are expressed by various cell types in the human tuberculous lung, and their expression patterns are reflected by differences in the immune response.