Effect of local hyperthermia on lymphangiogenic factors VEGF-C and -D in a nude mouse xenograft model of tongue squamous cell carcinoma
Effect of local hyperthermia on lymphangiogenic factors VEGF-C and -D in a nude mouse xenograft model of tongue squamous cell carcinoma
复制标题
局部热疗对舌鳞癌裸鼠移植瘤模型淋巴管生成因子VEGF-C和-D的影响
DOI：
10.1016/j.oraloncology.2009.12.001
复制
发表时间：
2010-02-01
期刊：
影响因子：
4.8
通讯作者：
Yang, Jing
中科院分区：
文献类型：
--
作者：
Liang, Xinhua;Zhou, Hao;Yang, Jing
血管内皮生长因子C和/或血管内皮细胞生长因子D诱导的血管内皮生长因子受体3的激活可促进肿瘤淋巴管的生成，从而促进淋巴转移。本研究观察了舌鳞状细胞癌裸鼠模型局部热疗后肿瘤生长及瘤内血管内皮生长因子C和血管内皮生长因子D的表达水平。结果表明，局部热疗组肿瘤体积为对照组的26.5%，实时荧光定量RT-PCR、Western印迹和免疫组织化学检测结果显示，局部热疗组肿瘤组织中VEGF-C和-D的表达明显受到抑制(P<；0.05)。这些结果提示，局部热疗在抑制肿瘤生长的同时，可能通过抑制肿瘤血管内皮生长因子-C和-D的表达而发挥抗淋巴管生成的作用，从而抑制舌鳞癌癌细胞的淋巴转移。(C)2009爱思唯尔有限公司。保留所有权利。
It is widely accepted that tumor-induced lymphangiogenesis driven by vascular endothelial growth factor (VEGF)-C- and/or VEGF-D-induced activation of VEGF receptor (VEGFR)-3 could promote lymphatic metastasis. In this study, tumor growth and intratumoral expression level of VEGF-C and -D following administration of local hyperthermia was evaluated in nude mice model of tongue squamous cell carcinoma (SCC). The data demonstrated that the size of tumor in local hyperthermia was 26.5% of control group, and that local hyperthermia markedly suppressed the mRNA and protein expression of VEGF-C and -D as determined by quantitative real-time RT-PCR, Western blot and immunohistochemistry (P < 0.05). These results suggest that, accompanying with tumor growth inhibition, local hyperthermia may act as an anti-lymphangiogenic role by suppressing the expression of tumor VEGF-C and -D, and thereby inhibiting cancer cell lymphatic metastasis in tongue SCC. (C) 2009 Elsevier Ltd. All rights reserved.