High follicle-stimulating hormone increases aneuploidy in human oocytes matured in vitro
High follicle-stimulating hormone increases aneuploidy in human oocytes matured in vitro
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高卵泡刺激激素增加体外成熟的人卵母细胞的非整倍性
DOI：
10.1016/j.fertnstert.2010.04.037
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发表时间：
2011-01-01
影响因子：
6.7
通讯作者：
Zhou, Can-Quan
中科院分区：
文献类型：
--
作者：
Xu, Yan-Wen;Peng, Yue-Ting;Zhou, Can-Quan
目的：研究FSH对体外成熟的人卵母细胞非整倍体风险的影响。设计：前瞻性研究。设置：医院IVF中心。患者：接受卵胞浆内单精子注射（ICSI）周期的男性因素不育患者。干预：根据体外成熟培养液中FSH浓度（0、5.5、22、100和2,000 ng/mL）将未成熟卵母细胞分为5组。在偏光显微镜下观察病灶，然后进行极体活检。用荧光原位杂交技术检测了13、16、18、21和22号染色体上的固定极体和相应的卵母细胞。结果：5个FSH组的非整倍体发生率分别为26.7%、23.3%、36.75%、46.67%和63.3%。在2,000 ng/mL FSH组中，成熟卵母细胞的非整倍体率显著较高。在PolScope下评估的纺锤体可视化率在非整倍体和正常卵母细胞之间没有显著差异。2,000和5.5 ng/mL FSH组的纺锤体形态无差异。结论：高浓度FSH的IVM培养液显著增加了第一次减数分裂错误率，导致IVM过程中非整倍体卵母细胞增多。（Fertil Steril（R）2011; 95：99-104.（C）2011年由美国生殖医学协会。
Objective: To study the effect of FSH on the aneuploidy risk of human oocytes matured in vitro.Design: Prospective study.Setting: Hospital-based IVF center.Patient(s): Patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles.Intervention(s): Immature oocytes were put into five groups according to the FSH concentration (0, 5.5, 22, 100, and 2,000 ng/mL) in in vitro maturation (IVM) medium. Spindles were observed under a polarized microscope before polar body biopsy. Fixed polar bodies and corresponding oocytes were examined on chromosomes 13, 16, 18, 21, and 22 by fluorescence in situ hybridization. Oocytes matured in 5.5 and 2,000 ng/mL FSH were immunostained for tubulin and chromatin.Main Outcome Measure(s): Aneuploidy rate, spindle visualization rate, and spindle morphology.Result(s): The frequency rates of aneuploidy were 26.7%, 23.3%, 36.75%, 46.67%, and 63.3% in the five FSH groups, respectively. There was a significantly higher aneuploidy rate in oocytes matured in the 2,000 ng/mL FSH group. The spindle visualization rates assessed under PolScope were not significantly different between aneuploid and normal oocytes. There was no difference in spindle morphology between the 2,000 and 5.5 ng/mL FSH groups.Conclusion(s): High-concentration FSH in IVM medium significantly increased the first meiotic division error, resulting in more aneuploid oocytes during IVM. (Fertil Steril (R) 2011; 95: 99-104. (C)2011 by American Society for Reproductive Medicine.)