Towards multi-level biomonitoring of nematodes to assess risk of nitrogen and phosphorus pollution in Jinchuan Wetland of Northeast China
Towards multi-level biomonitoring of nematodes to assess risk of nitrogen and phosphorus pollution in Jinchuan Wetland of Northeast China
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DOI：
10.1007/s10646-015-1550-5
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发表时间：
2015-09
期刊：
影响因子：
2.7
通讯作者：
Yunbiao Wang;Jiejuan Qiao;Chunguang He;Zhongqiang Wang;Wenbo Luo;L. Sheng
中科院分区：
文献类型：
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作者：
Yunbiao Wang;Jiejuan Qiao;Chunguang He;Zhongqiang Wang;Wenbo Luo;L. Sheng
农业生产耕作往往对附近肥沃地貌的湿地生态系统构成威胁。本研究采用随机抽样的方法，通过对金川湿地湿地、农田及湿地和农田边缘的主要土壤性质的研究，对金川湿地线虫生态指标进行了评价。还研究了线虫在采样水中的行为和繁殖情况。应激蛋白Hsp70和Hsp90在雅致线虫的活体野外样本和实验室测试的线虫样本中都被检测到。我们的结果表明，氮和磷对湿地生态系统的干扰降低了线虫的丰富度和细菌体线虫的比例。细菌线虫多样性和植物寄生生态指数是湿地生态健康的敏感指标。线虫Hsp70是一种有用的生物传感器，可用于监测和评估湿地的氮磷污染水平。典型对应分析的多层次土壤动物评价结果表明，金川湿地受到附近农田非点源污染的威胁。
Cultivation for agricultural production often poses threats to nearby wetlands ecosystems in fertile landscapes. In this study, nematode ecological indexes were assessed through the main soil properties of the wetlands, farmlands, and edges of wetlands and farmlands in Jinchuan Wetland by the random sampling. Behavior and reproduction inCaenorhabditiselegans(C.elegans) exposed to the sampled waters were also examined. Stress proteins Hsp70 and Hsp90 were measured both in the living field samples ofC.elegansand the lab-testedC.elegans. Our results suggested that disturbance to wetland ecosystems by nitrogen and phosphorus reduced nematode richness and proportions of bacterivore nematodes. Bacterivore nematode diversity and plant–parasitic ecological index were proven to be sensitive indicators of the ecological health of wetlands. Nematode Hsp70 were useful biosensors to monitor and assess the levels of nitrogen and phosphorus pollutions in wetlands. Furthermore, multi-level soil faunal assessments by canonical correspondence analysis showed that Jinchuan Wetland is threatened with non-point source pollution from nearby farmlands.