Hyperthermia suppresses the cytotoxicity of NK cells via down-regulation of perforin/granzyme B expression
Hyperthermia suppresses the cytotoxicity of NK cells via down-regulation of perforin/granzyme B expression
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DOI：
10.1016/j.bbrc.2005.09.184
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发表时间：
2005-12-02
影响因子：
3.1
通讯作者：
Kai, H
中科院分区：
文献类型：
--
作者：
Koga, T;Harada, H;Kai, H
高热。作为癌症的辅助疗法，已知在体外可以调节自然杀伤（NK）细胞的活性，但其在体内的作用尚不清楚。在本研究中，我们采用红外线加热全身热疗（WBH）装置来评估全身热疗对小鼠模型的影响。我们在这里证明，野生型C57BL/6J小鼠暴露在42℃下60分钟，通过细胞溶解实验发现NK细胞对YAC-1靶细胞的溶解活性降低。这一结果在Rag-2敲除小鼠中得到证实，这些小鼠具有功能性NK细胞，但不具有细胞溶解性T细胞或NK-T细胞。此外，白藜芦醇还能降低小鼠脾脏中perform和颗粒酶B mRNA的表达。但TNF细胞因子（Fas配体和TRAIL）的表达没有变化。这些数据表明，白骨精对NK细胞活性的抑制可能通过穿孔素/颗粒酶途径介导。(c) 2005爱思唯尔公司版权所有。
Hyperthermia. which is used as an adjunctive therapy for cancer, is known to modulate the activity of natural killer (NK) cells in vitro, but its effect in vivo is unclear. In the present study, we used a whole body hyperthermia (WBH) device heated by infrared rays to evaluate the effect of WBH on mice models. We demonstrate here that wild type C57BL/6J mice exposed to 42 degrees C for 60 min had reduced NK cell cytolytic activity against YAC-1 target cells as determined by cytolytic assay. This result was confirmed using Rag-2 knockout mice, which possess functional NK but not cytolytic T or NK-T cells. Moreover, WBH decreased the mRNA expression of perform and granzyme B in spleens of mice. But the expression of TNF cytokines (Fas ligand and TRAIL) was unchanged. These data suggest that the suppression of NK cell activity induced by WBH Could be mediated through the perforin/granzyme pathway. (c) 2005 Elsevier Inc. All rights reserved.