A self-assembling peptide polynanoreactor
A self-assembling peptide polynanoreactor
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DOI：
10.1002/anie.200604014
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发表时间：
2007-01-01
影响因子：
16.6
通讯作者：
Ryadnov, Maxim G.
中科院分区：
文献类型：
--
作者：
Ryadnov, Maxim G.
Biomolecular self-assembly has been increasingly applied to the fabrication of novel supramolecular structures.[1] The approach allows the construction of a supramolecular composite or its components from the bottom up, a route that offers the potential to define and control the properties of the resulting materials at the nanometer scale. Nanoreactors particularly benefit from this, as their key function—deposition of a reaction or a material—is intimately associated with confining a reaction environment.[2] It becomes even more critical in designing a nanoreactor with multiple reaction sites, which has yet to be reported. Herein, a new design is described that allows the amplification of a discrete material within an individual nanoreactor; that is, it acts as a “polynanoreactor”. The system results from a noncovalent dendrimer-like assembly of short leucine zipper sequences.[3, 4] The designed polynanoreactors host numerous nanosized cavities that are demonstrated to function as encapsulating sites in casting copious uniformly sized silver nanoparticles. A prerequisite for all types of nanoreactors is encapsulation,[2] that is, an ability to form and sustain cavities capable of hosting or encapsulating guest materials. Designing a nanoreactor with multiple cavities introduces a higher level of complexity, which eventually determines the choice of a molecular candidate. One molecular class that meets these requirements is self-assembled dendrimers, which have been extensively exploited over the last two decades.[5, 6] Pioneering studies by Zimmerman et al.,[7] Percec et al.,[8] Tomalia and co-workers,[9] Hecht and FrØchet,[10] and Meijer and coworkers [11] on supramolecular dendrimer systems offer a strong basis to apply the properties of the dendritic state to engineering nanoreactors. Most remarkably, the properties of such systems are reminiscent of those displayed by natural nanoreactors such as ribosomes, enzyme complexes, and protein cages.[2] Therefore, the main drive of the present study in designing polynanoreactors is to express dendrimer architectures by a “natural” self-assembly motif.The polynanoreactors were designed in two steps. The first step consisted of building a noncovalent dendrimer framework, which is referred to herein as a supradendrimer (SD). This SD is obtained from the self-assembly of a single peptide sequence, SD-1 (Figure 1A), which is based on a dimeric coiled coil also known as the leucine zipper. It is one of the most abundant assembling motifs in natural biosystems and presents a bundle of two α helices.[3] Amino acid preferences that distinguish between the character and oligomerization state of the bundle can be rationally specified
