Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9
Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9
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DOI：
10.1016/j.ibmb.2014.03.010
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发表时间：
2014-06-01
影响因子：
3.8
通讯作者：
Xia, Qingyou
中科院分区：
文献类型：
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作者：
Liu, Yuanyuan;Ma, Sanyuan;Xia, Qingyou
海蛞蝓是一种重要的经济昆虫，也是研究鳞翅目和节肢动物生物学的模式生物。使用高效的CRISPR/Cas9系统，我们表明该系统可以介导单个基因座的高效靶向基因组编辑，大的染色体缺失或倒位，以及在源自B的BmNs细胞系中同时介导6个基因的多重基因组编辑。桑。我们的系统的简单性和高效率为研究人员提供了前所未有的可能性，以实现精确和复杂的操作所选择的B。mori基因在BmNs细胞中的表达，为B的功能基因组学研究提供了新的契机。桑和其他鳞翅目昆虫。(C)2014爱思唯尔有限公司版权所有。
Bombyx mari is an economically important insect and a model organism for studying lepidopteran and arthropod biology. Using a highly efficient CRISPR/Cas9 system, we showed that this system could mediate highly efficient targeted genome editing of a single gene locus, large chromosomal deletion or inversion, and also multiplex genome editing of 6 genes simultaneously in BmNs cell line derived from B. mori. The simplicity and high efficiency of our system provide unprecedented possibilities for researchers to implement precise and sophisticated manipulation of a chosen B. mori gene in BmNs cells easily in a limited time course, and perhaps new opportunities for functional genomics of B. mori and other lepidopteran insects. (C) 2014 Elsevier Ltd. All rights reserved.