REGULARLY SCHEDULED VOLUNTARY EXERCISE SYNCHRONIZES THE MOUSE CIRCADIAN CLOCK
REGULARLY SCHEDULED VOLUNTARY EXERCISE SYNCHRONIZES THE MOUSE CIRCADIAN CLOCK
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DOI：
10.1152/ajpregu.1991.261.4.r928
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发表时间：
1991-10-01
影响因子：
--
通讯作者：
DEMENT, WC
中科院分区：
文献类型：
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作者：
EDGAR, DM;DEMENT, WC
长期以来，人们一直认为昼夜节律的诱导完全依赖于外部环境中的周期性线索。 然而，现在的证据表明，适当时机的剧烈活动也可能使生物钟发生相移。 以前不知道与自发行为相关的运动/活动水平是否提供了足够的反馈来相移或同步昼夜节律。 本研究通过监测小鼠（小家鼠）在不受限制地使用跑步轮期间的睡眠-觉醒、饮水和跑步轮昼夜节律以及自由轮旋转被限制为12小时或6小时间隔（固定周期为24小时）来调查这一问题。 轮子的转动是远程控制的。 小鼠在预定的访问过程中自发地跑在轮子上，15只动物中有11只动物的自由奔跑的睡眠-觉醒和饮水昼夜节律被带入预定的运动。 然而，只有当运动开始几个小时到主观的夜晚，达到稳态夹带。 牵拉过程中跑步的时间位置与牵拉前的自由跑步时间相关（r = 0.7003，P < 0.02）。 自由奔跑时间< 23.0 h的小鼠不进行牵引，但表现出自由奔跑变量与车轮可用性时间表之间的相对协调。 因此，昼夜节律计时系统响应于由意志运动/活动的定时产生的时间反馈，这表明生物钟不仅响应于外部环境中的周期性地球物理事件，而且还从生物体的自发活动行为中获得生理反馈。
Circadian rhythm entrainment has long been though to depend exclusively on periodic cues in the external environment. However, evidence now suggests that appropriately timed vigorous activity may also phase shift the circadian clock. Previously it was not known whether levels of exercise/activity associated with spontaneous behavior provided sufficient feedback to phase shift or synchronize circadian rhythms. The present study investigated this issue by monitoring the sleep-wake, drinking, and wheel-running circadian rhythms of mice (Mus musculus) during unrestricted access to running wheels and when free wheel rotation was limited to either 12- or 6-h intervals with a fixed period of 24 h. Wheel rotation was controlled remotely. Mice spontaneously ran in wheels during scheduled access, and free-running sleep-wake and drinking circadian rhythms became entrained to scheduled exercise in 11 of 15 animals. However, steady-state entrainment was achieved only when exercise commenced several hours into the subjective night. The temporal placement of running during entrainment was related (r = 0.7003, P < 0.02) to free-running period before entrainment. Mice with a free-running period < 23.0 h did not entrain but exhibited relative coordination between free-running variables and the wheel availability schedule. Thus the circadian timekeeping system responds to temporal feedback arising from the timing of volitional exercise/activity, suggesting that the biological clock not only is responsive to periodic geophysical events in the external environment but also derives physiological feedback from the spontaneous activity behaviors of the organism.