Synergistic effect of type and concentration of surfactant and diluting solvent on the morphology of emulsion templated matrices developed as tissue engineering scaffolds
Synergistic effect of type and concentration of surfactant and diluting solvent on the morphology of emulsion templated matrices developed as tissue engineering scaffolds
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DOI：
10.1016/j.reactfunctpolym.2022.105387
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发表时间：
2022-09-13
影响因子：
5.1
通讯作者：
Claeyssens, Frederik
中科院分区：
文献类型：
--
作者：
Dikici, Betul Aldemir;Dikici, Serkan;Claeyssens, Frederik
乳液模板是制备组织工程支架的一种有利途径。乳剂大多使用表面活性剂来稳定，表面活性剂的性能取决于各种参数，如乳化温度和电解质的存在。在本研究中，我们认为稀释溶剂类型对表面活性剂的效率和聚合乳液的形态也有很大的影响。为此，本课题组研究了用四种不同的表面活性剂和三种不同的溶剂制备的聚己内酯甲基丙烯酸酯聚合乳液的形态，该乳液专为组织工程应用和体外生物相容性而设计。结果表明，乳液组成中使用的稀释溶剂对表面活性剂的性能有很大影响，从而影响聚合乳液的形态。增加表面活性剂浓度和稀释溶剂体积对乳状液的特性有相反的影响。可以制备平均孔径为10 ~ 78 μ m的支架。建立这些关系使我们能够控制聚合乳液的整体形态，并通过调整溶剂和表面活性剂的类型和浓度，精确地设计它们用于特定的组织工程应用。
Emulsion templating is an advantageous route for the fabrication of tissue engineering scaffolds. Emulsions are mostly stabilised using surfactants, and the performances of the surfactants depend on various parameters such as emulsification temperature and the presence of the electrolytes. In this study, we suggest that diluting solvent type also has a dramatic impact on the efficiency of the surfactant and morphology of the polymerised emulsions. For this, morphologies of polycaprolactone methacrylate-based polymerised emulsions, which are designed for tissue engineering applications and in vitro biocompatibilities, were shown by our group, prepared using four different surfactants, and three different solvents were investigated. Results showed that the diluting solvent used in the emulsion composition has a strong impact on the performance of the surfactant and consequently on the morphology of polymerised emulsions. Increasing surfactant concentration and diluting solvent volume have an opposite impact on the characteristics of emulsions. Scaffolds with average pore sizes changing from 10 to 78 mu m could be fabricated. Establishing these relations enables us to have control over the overall morphology of polymerised emulsions and precisely engineer them for specific tissue engineering applications by tuning solvent and surfactant type and concentration.