EVIDENCE THAT TRANSFORMING GROWTH-FACTOR-BETA IS A HORMONALLY REGULATED NEGATIVE GROWTH-FACTOR IN HUMAN-BREAST CANCER-CELLS
EVIDENCE THAT TRANSFORMING GROWTH-FACTOR-BETA IS A HORMONALLY REGULATED NEGATIVE GROWTH-FACTOR IN HUMAN-BREAST CANCER-CELLS
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DOI：
10.1016/0092-8674(87)90193-0
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发表时间：
1987-02-13
期刊：
影响因子：
64.5
通讯作者：
DICKSON, RB
中科院分区：
文献类型：
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作者：
KNABBE, C;LIPPMAN, ME;DICKSON, RB
激素依赖性人乳腺癌细胞系MCF-7分泌转化生长因子-.β。 (TGF-β)，可以在培养基中以生物活性形式检测到。这些多肽与人血小板衍生的TGF-β竞争。由于与其受体结合，它们在TGF-β特异性生长测定中具有生物活性，并且被TGF-β特异性抗体识别和灭活。活性TGF-β的分泌。在用生长抑制浓度的抗雌激素处理 MCF-7 细胞时，可诱导 8 至 27 倍。抗雌激素诱导的TGF-β。在共培养实验中，来自 MCF-7 细胞的 MCF-7 细胞抑制雌激素受体阴性人乳腺癌细胞系的生长；生长抑制可以用抗TGF-β逆转。抗体。我们得出结论，在 MCF-7 细胞中，TGF-β。是一种激素调节的生长抑制剂，在乳腺癌细胞中具有可能的自分泌和旁分泌功能。
The hormone-dependent human breast cancer cell line MCF-7 secretes transforming growth factor-.beta. (TGF-.beta.), which can be detected in the culture medium in a biologically active form. These polypeptides compete with human platelet-derived TGF-.beta. for binding to its receptor, are biologically active in TGF-.beta.-specific growth assays, and are recognized and inactivated by TGF-.beta.-specific antibodies. Secretion of active TGF-.beta. is induced 8 to 27-fold under treatment of MCF-7 cells with growth inhibitory concentrations of antiestrogens. Antiestrogen-induced TGF-.beta. from MCF-7 cell inhibits the growth of an estrogen receptor-negative human breast cancer cell line in coculture experiments; growth inhibition is reversed with anti-TGF-.beta. antibodies. We conclude that in MCF-7 cells, TGF-.beta. is a hormonally regulated growth inhibitor with possible autocrine and paracrine functions in breast cancer cells.