PLURIPOTENT TERATOCARCINOMA-THYMUS SOMATIC-CELL HYBRIDS
PLURIPOTENT TERATOCARCINOMA-THYMUS SOMATIC-CELL HYBRIDS
复制标题
DOI：
10.1016/0092-8674(76)90051-9
复制
发表时间：
1976-01-01
期刊：
影响因子：
64.5
通讯作者：
RUDDLE, FH
中科院分区：
文献类型：
--
作者：
MILLER, RA;RUDDLE, FH
通过将多能性PCC 4aza 1胚胎癌（畸胎癌）细胞与来自年轻成年小鼠的胸腺细胞融合产生一系列体细胞杂种。当这些杂交体在nu/nu或同基因小鼠中形成肿瘤时，所有肿瘤都含有一系列分化组织以及胚胎癌样组织。一些肿瘤产生α-胎儿蛋白胚胎癌细胞的多能性不需要通过从分化细胞引入完整的二倍体基因组来消除。
A series of somatic cell hybrids was produced by fusing pluripotent PCC4aza1 embryonal carcinoma (teratocarcinoma) cells with thymocytes from young adult mice. When these hybrids form tumors in nu/nu or syngeneic mice, all tumors contain a range of differentiated tissues, as well as embryonal carcinoma-like tissue. Some of the tumors produce .alpha.-fetoprotein. Pluripotency in embryonal carcinoma cells need not be abolished by the introduction of a complete diploid genome from a differentiated cell.