Cellular lipidomics
Cellular lipidomics
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DOI：
10.1038/sj.emboj.7600798
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发表时间：
2005-09-21
期刊：
影响因子：
11.4
通讯作者：
van Meer, G
中科院分区：
文献类型：
--
作者：
van Meer, G
细胞脂质组包含超过1000种不同的脂质。大多数脂质看起来相似，具有极性头部和疏水性尾部。尽管如此，细胞识别脂质的特异性仍然很高。脂质的功能性由其局部浓度决定，其在细胞器之间、在脂质双层的两个小叶之间甚至在膜的侧平面内变化。为了整合功能，细胞脂质组学不仅必须确定存在哪些脂质，而且还必须确定每种脂质在每个特定细胞内位置的浓度以及脂质的相互作用伴侣。此外，细胞脂质组学必须包括脂质代谢和转运的酶，它们的特异性，定位和调控。最后，它需要对脂质和膜的物理性质，特别是脂质-脂质和脂质-蛋白质相互作用的透彻理解。在细胞中，代谢物之间的复杂关系只能通过将它们视为一个完整的系统来理解。细胞脂质组学为理解和操纵脂质的重要作用提供了一个框架，特别是在膜转运和分选以及细胞信号传导中。
The cellular lipidome comprises over 1000 different lipids. Most lipids look similar having a polar head and hydrophobic tails. Still, cells recognize lipids with exquisite specificity. The functionality of lipids is determined by their local concentration, which varies between organelles, between the two leaflets of the lipid bilayer and even within the lateral plane of the membrane. To incorporate function, cellular lipidomics must not only determine which lipids are present but also the concentration of each lipid at each specific intracellular location in time and the lipid's interaction partners. Moreover, cellular lipidomics must include the enzymes of lipid metabolism and transport, their specificity, localization and regulation. Finally, it requires a thorough understanding of the physical properties of lipids and membranes, especially lipid - lipid and lipid - protein interactions. In the context of a cell, the complex relationships between metabolites can only be understood by viewing them as an integrated system. Cellular lipidomics provides a framework for understanding and manipulating the vital role of lipids, especially in membrane transport and sorting and in cell signaling.