Integration-Dependent Bacteriophage Immunity Provides Insights into the Evolution of Genetic Switches
Integration-Dependent Bacteriophage Immunity Provides Insights into the Evolution of Genetic Switches
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DOI：
10.1016/j.molcel.2012.11.012
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发表时间：
2013-01-24
期刊：
影响因子：
16
通讯作者：
Hatfull, Graham F.
中科院分区：
文献类型：
--
作者：
Broussard, Gregory W.;Oldfield, Lauren M.;Hatfull, Graham F.
基因开关是发育回路的关键组成部分。由于温带噬菌体种类繁多，种类繁多，它们为了解开关的机制和进化以及遗传回路的分子控制提供了丰富的资源。在这里，我们描述了一种新的小型、紧凑和简单的交换机，它使用特定于站点的重组作为关键决策点。噬菌体附着位点attP位于噬菌体抑制子基因内，因此染色体整合导致C末端标签的移除，该标签破坏了病毒编码形式的抑制子的稳定性。因此，整合不仅赋予原噬菌体稳定性，也是建立溶原性的必要条件。这些独立的整合依赖的免疫系统在不同的基因组环境中的多样性表明，这些系统代表了开关进化的祖先状态，更复杂的开关是从这些祖先进化而来的。它们还为构建合成生物电路提供了一个强大的工具包。
Genetic switches are critical components of developmental circuits. Because temperate bacteriophages are vastly abundant and greatly diverse, they are rich resources for understanding the mechanisms and evolution of switches and the molecular control of genetic circuitry. Here, we describe a new class of small, compact, and simple switches that use site-specific recombination as the key decision point. The phage attachment site attP is located within the phage repressor gene such that chromosomal integration results in removal of a C-terminal tag that destabilizes the virally encoded form of the repressor. Integration thus not only confers prophage stability but also is a requirement for lysogenic establishment. The variety of these self-contained integration-dependent immunity systems in different genomic contexts suggests that these represent ancestral states in switch evolution from which more-complex switches have evolved. They also provide a powerful toolkit for building synthetic biological circuits.