Organization of RFLP diversity among inbred lines of maize representing the most significant heterotic groups
Organization of RFLP diversity among inbred lines of maize representing the most significant heterotic groups
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DOI：
10.2135/cropsci1996.0011183x003600030043x
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发表时间：
1996-05-01
期刊：
影响因子：
2.3
通讯作者：
Charcosset, A
中科院分区：
文献类型：
--
作者：
Dubreuil, P;Dufour, P;Charcosset, A
116个玉米自交系（Zea mays L.）来自显著的杂种优势群（即，欧洲燧石，兰开斯特肯定作物，明尼苏达州13，和里德黄凹痕）和其他来源进行了限制性片段长度多态性（RFLP）分析。我们的目标是调查（i）遗传变异及其结构之间和杂种优势群内和（ii）利用RFLP标记分配杂线杂种优势群的探针选择莲花特异性，清晰的模式，和再现性。在使用的63个单拷贝探针-酶组合中，对372个变体进行评分，每个组合平均5.9个变体。主成分和聚类分析从建立的杂种优势群行明确区分提示线从凹痕线。确定了对欧洲燧石和北方美国凹痕品系之间的遗传分化贡献最大的基因座。在美国的种质，分类显示了密切匹配的协会基于系谱关系的近亲繁殖的协会。家系距离与分子Rogers'距离呈显著正相关（r = 0.77，P < 0.001）。基于这些结果，分子距离分类是方便的（i）鉴定杂种优势群和（ii）分配来源未知或广泛基础的自交系。这些信息应证明是有用的选择（i）在杂交试验中进行评估的自交系组合和（ii）育种计划的父母。
One hundred-sixteen inbred lines of maize (Zea mays L.) from significant heterotic groups (i.e., European flint, Lancaster Sure Crop, Minnesota 13, and Reid Yellow Dent) and miscellaneous origins were assayed for restriction fragment length polymorphism (RFLP). Our goals were to investigate (i) genetic variability and its structure among and within heterotic groups and (ii) the use of RFLP markers to assign miscellaneous lines to heterotic groups by means of probes selected for lotus specificity, clear patterns, and reproducibility. Among the 63 single copy probe-enzyme combinations used, 372 variants were scored with a mean of 5.9 variants per combination. Principal component and cluster analyses performed on lines from established heterotic groups clearly separated hint lines from dent lines. Loci that contributed the most to the genetic differentiation between European flint and northern U.S. dent lines were determined. Within U.S. germplasm, classification showed an association of inbreds that closely matched associations based on pedigree relationships. Correlation between pedigree distance and molecular Rogers' distance was significant (r = 0.77, P < 0.001). Based on these results, classification by molecular distance was convenient (i) for identifying heterotic groups and (ii) for assigning origins to unknown or broadly based inbreds. This information should prove useful for choosing (i) combinations of inbreds to be evaluated in hybrid trials and (ii) parents for breeding programs.