Trichinella nativa and Trichinella T9 in the Hokkaido island, Japan
Trichinella nativa and Trichinella T9 in the Hokkaido island, Japan
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DOI：
10.1016/j.parint.2006.08.004
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发表时间：
2006-12-01
影响因子：
1.9
通讯作者：
Oku, Yuzaburo
中科院分区：
文献类型：
--
作者：
Kanai, Yuta;Nonaka, Nariaki;Oku, Yuzaburo
旋毛虫属肌肉幼虫是从 2003 年在日本北海道札幌和音更捕获的两只红狐 (Vulpes vulpes) 的大腿肌肉中分离出来的。为旋毛虫属基因分型而设计的多重 PCR 显示，札幌分离株显示出针对 T. britovi 复合体（T. britovi、旋毛虫 T8 和旋毛虫 T9）的特定模式，而音更分离株则显示出针对旋毛虫属的特定模式。原生。札幌分离株的线粒体细胞色素氧化酶亚基 I (COI) 基因的一部分和内转录间隔区 2 (ITS2) 的核苷酸序列与在日本大陆检测到的旋毛虫 T9 的相似性最高。这项研究表明，T nativa 和 T9 旋毛虫均在北海道岛的野生动物中传播。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
Trichinella sp. muscle larvae were isolated from the thigh muscle of two red foxes (Vulpes vulpes) captured in Sapporo and Otofuke, Hokkaido, Japan, in 2003. Multiplex PCR designed for genotyping the genus Trichinella revealed that the Sapporo isolate showed a specific pattern to T. britovi complex (T britovi, Trichinella T8 and Trichinella T9) and the Otofuke isolate showed that to T. nativa. Nucleotide sequences of a part of the mitochondrial cytochrome oxidase subunit I (COI) gene and internal transcribed spacer 2 (ITS2) of the Sapporo isolate showed the highest similarity to those of Trichinella T9, a species detected in the mainland of Japan. This study shows that both T nativa and Trichinella T9 are circulating in wildlife of the Hokkaido island. (c) 2006 Elsevier Ireland Ltd. All rights reserved.