Insulin treatment increases skeletal muscle fibre area in patients with diabetes mellitus type 2
Insulin treatment increases skeletal muscle fibre area in patients with diabetes mellitus type 2
复制标题
DOI：
10.1046/j.1365-2281.2000.00269.x
复制
发表时间：
2000-09-01
期刊：
影响因子：
--
通讯作者：
Jansson, E
中科院分区：
文献类型：
--
作者：
Cederholm, T;Sylvén, C;Jansson, E
观察了18例2型糖尿病控制不良(平均病程7.5±6年)患者(62±11岁)胰岛素治疗对骨骼肌功能的影响。在胰岛素治疗前和治疗后40+/-14天，分别从股四头肌外侧部分采集骨骼肌活检样本。测定酶活性(磷酸果糖激酶、3-羟基乙酰辅酶A脱氢酶、柠檬酸合成酶、乳酸脱氢酶和肌酸激酶)和肌红蛋白含量。在11例患者(60+/-11岁)中，还分析了骨骼肌纤维类型组成(I型、IIA型、IIB型和IIC型)和纤维型横截面积。胰岛素治疗后，IIA型和IIB型快速收缩纤维的横截面积分别增加了32%和38%(P<；0.02)。纤维类型分布没有改变。肌肉中肌红蛋白含量降低20%(P<；0.01)。在所测试的酶中，3-羟基酰辅酶A脱氢酶活性降低了10%(P<；0.04)。血糖、HbA1(C)和血清甘油三酯水平降低(P<；0.001)，体重和上臂肌围增加(P<；0.02)。总而言之，对控制不佳的非胰岛素依赖型糖尿病患者进行胰岛素治疗后，快抽动纤维面积增加，肌红蛋白水平降低，与脂肪酸氧化相关的肌酶活性降低。
The effects of insulin treatment on skeletal muscle characteristics were studied in 18 patients (62 +/- 11 years) with poorly controlled diabetes mellitus type 2 (mean duration 7.5 +/- 6 years). Skeletal muscle biopsy samples were taken from the lateral portion of the quadriceps muscle before and after a period of insulin treatment of 40 +/- 14 days. Enzyme activities (phosphofructokinase, 3-hydroxyacyl-CoA dehydrogenase, citrate synthase, lactate dehydrogenase and creatine kinase) and myoglobin content were assessed. In a subgroup of 11 patients (60 +/- 11 years), skeletal muscle fibre type composition (type I, IIA, IIB and IIC) and fibre type cross-sectional area were also analysed. Following insulin treatment there were 32 and 38% increases, respectively, in the cross-sectional areas of type IIA and IIB fast-twitch fibres (P < 0.02). The fibre type distribution did not change. The myoglobin content in muscle decreased by 20% (P < 0.01). Of the enzymes tested, the 3-hydroxyacyl-CoA dehydrogenase activity decreased by 10% (P < 0.04). Serum glucose, HbA1(C) and serum triglyceride levels decreased (P < 0.001) and body weight and arm muscle circumference increased (P < 0.02). In conclusion, insulin treatment of patients with poorly controlled non-insulin-dependent diabetes mellitus increased the fast-twitch fibre area, reduced myoglobin levels and decreased muscle enzyme activity related to fatty acid oxidation.