OLFACTORY SCHWANN-CELLS ARE DERIVED FROM PRECURSOR CELLS IN THE OLFACTORY EPITHELIUM
OLFACTORY SCHWANN-CELLS ARE DERIVED FROM PRECURSOR CELLS IN THE OLFACTORY EPITHELIUM
复制标题
DOI：
10.1002/jnr.490290206
复制
发表时间：
1991-06-01
影响因子：
4.2
通讯作者：
AU, C
中科院分区：
文献类型：
--
作者：
CHUAH, MI;AU, C
在形态学和免疫组化研究中，我们发现嗅觉雪旺细胞（OSC）来源于嗅觉上皮内的前体细胞。在发育过程中，它们从上皮中迁移出来并延伸到嗅觉轴突。用胰蛋白酶-胰酶处理E14大鼠胚胎和幼年大鼠嗅粘膜，去除下层结缔组织。然后上皮外植体在培养中保持两天，在此期间细胞从外植体中迁移出来。其中细长双极细胞对胶质原纤维酸性蛋白、超微结构及与生长轴突的关联呈阳性免疫反应，鉴定为OSC。在胚胎外植体中，轴突生长更为丰富，OSC的极性与轴突平行。超微结构观察显示，轴突束的鞘层与体内相似。
In this morphological and immunohistochemical study we show that olfactory schwann cells (OSC) are derived from precursor cells residing in the olfactory epithelium. During development, they migrate out of the epithelium and extend processes to ensheath the olfactory axons.Olfactory mucosa from E14 rat embryos and juvenile rats were treated with trypsin-pancreatin to remove the underlying connective tissue. The epithelial explant was then maintained for two days in culture, during which cells migrated out from the explant. Among them were spindly bipolar cells which were identified as OSC by their positive immunoreaction for glial fibrillary acidic protein, ultrastructure, and association with growing axons. Axonal growth was significantly more profuse in the embryonic explants, in which the polarity of the OSC was oriented parallel with the axons. Ultrastructural observations showed that ensheathment of the bundles of axons resembled those in vivo.