Fabrication and characterization of aligned nanofibrous PLGA/Collagen blends as bone tissue scaffolds
Fabrication and characterization of aligned nanofibrous PLGA/Collagen blends as bone tissue scaffolds
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DOI：
10.1016/j.polymer.2009.05.035
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发表时间：
2009-07-17
期刊：
影响因子：
4.6
通讯作者：
Nyairo, Elijah
中科院分区：
文献类型：
--
作者：
Jose, Moncy V.;Thomas, Vinoy;Nyairo, Elijah
采用静电纺丝法制备了不同PLGA /胶原组分（80/20、65/35和50/50）的聚乳酸-羟基乙酸（PLGA）与胶原的排列纳米纤维共混物，并对其骨组织工程性能进行了表征。形态学表征表明，加入胶原蛋白后，PLGA的直径分布变窄，平均直径减小。差示扫描量热法（DSC）研究表明，天然胶原蛋白的三螺旋结构在制备过程中没有被破坏。然而，共混对共混物的总焓有显著的影响，总焓随着胶原蛋白含量的降低而降低。热重分析表明，胶原蛋白的加入提高了支架的亲水性。用1-乙基-3-（3-二甲氨基丙基）盐酸碳二亚胺（EDC）在乙醇中进行胶原交联以提高生物稳定性，总体交联度接近25%。EDC交联对80/20共混体系的纳米纤维形貌影响不大；然而，在较高的胶原浓度下，纳米纤维特征在一定程度上受到损害。干湿条件下的力学特性分析表明，胶原含量的增加导致其力学性能的显著下降。然而，交联导致湿式PLGA/胶原蛋白80/20共混体系的弹性模量从47 MPa增加到83 MPa，对拉伸强度影响不大。综上所述，本研究中使用的排列纳米纤维支架是一种很有前途的骨组织工程材料。2009爱思唯尔有限公司版权所有。
Aligned nanofibrous blends of Poly (D, L-lactide-co-glycolide) (PLGA) and collagen with various PLCA/ collagen compositions (80/20, 65/35 and 50/50) were fabricated by electrospinning and characterized for bone tissue engineering. Morphological characterization showed that the addition of collagen to PLGA resulted in narrowing of the diameter distribution and a reduction in average diameter. Differential scanning calorimetric (DSC) studies showed that the triple helix structure of the native collagen was not destroyed during the fabrication process. However, the blending had a marked effect on the overall enthalpy of the blends, whereby the total enthalpy decreased as the collagen content decreased. Thermogravimetric analysis showed the addition of collagen increased the hydrophilicity of the scaffolds. The crosslinking of collagen to increase the biostability was done using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in ethanol and an overall similar to 25% degree of crosslinking was achieved. The EDC crosslinking had little effect on the nanofibrous morphology of the 80/20 blend system; however, the nanofibrous features were compromised to some extent at higher collagen concentrations. The mechanical characterization under dry and wet conditions showed that increasing collagen content resulted in a tremendous decrease in the mechanical properties. However, crosslinking resulted in the increase in elastic modulus from 47 MPa to 83 MPa for the wet PLGA/Collagen 80/20 blend system, with little effect on the tensile strength. In conclusion, the aligned nanofibrous scaffold used in this study constitutes a promising material for bone tissue engineering. (C) 2009 Elsevier Ltd. All rights reserved.