Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay--assay validation for human milk.
Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay--assay validation for human milk.
复制标题
酶联免疫吸附测定母乳中二恶英毒性评价方法的建立——母乳测定验证。
DOI：
10.1016/s0045-6535(01)00267-3
复制
发表时间：
2002
期刊：
影响因子：
8.8
通讯作者：
Y. Matsuki
中科院分区：
文献类型：
--
作者：
Y. Sugawara;Koichi Saito;M. Ogawa;Susumu Kobayashi;G. Shan;J. Sanborn;B. Hammock;H. Nakazawa;Y. Matsuki
本研究建立了一种酶联免疫吸附试验（ELISA）检测人乳中二恶英类化合物毒性的方法。共17个人乳样本进行了酶联免疫吸附试验和气相色谱/质谱法（GC/MS）测试，以评估从母乳中提取的样本进行的酶联免疫吸附试验是否可以被认为是足够可靠的，以确定人乳中的二恶英污染。毒性当量（TEQ）筛选的概念进行了验证，通过比较TEQ值的人乳样品的ELISA反应预测这些样品。人乳的免疫测定与GC/MS法之间具有良好的相关性（r=0.920）。该方法可用于生物样品的检测。
In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r=0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.