ROLE OF BASAL LAMINA IN NEOPLASTIC DISORGANIZATION OF TISSUE ARCHITECTURE
ROLE OF BASAL LAMINA IN NEOPLASTIC DISORGANIZATION OF TISSUE ARCHITECTURE
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DOI：
10.1073/pnas.78.6.3901
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
JAMIESON, JD
中科院分区：
文献类型：
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作者：
INGBER, DE;MADRI, JA;JAMIESON, JD
研究了大鼠胰腺的可移植癌，其由细胞学分化的腺泡细胞组成，这些腺泡细胞已经失去了它们的上皮方向并且不形成腺泡。光学显微镜显示这些细胞在与脉管系统接触的区域中具有一致的栅栏、重定向和极化。EM显示正常基底层（BL）沿着复极化肿瘤细胞的基底部分，其与内皮BL物理分离。间接免疫荧光法被用来检查分布的BL成分，层粘连蛋白（LM）和IV型胶原（IV型）内的不同微环境的这种肿瘤。在正常胰腺中，Lm和IV型呈线性分布，勾勒出腺泡和血管的轮廓。在肿瘤实质中未检测到IV型，而Lm以点状分布出现，勾勒出细胞的轮廓。仅在沿着线性沉积的Lm和IV型边界血管观察到肿瘤细胞的重新定向。这种非转移性肿瘤在其紊乱的实质内明显丧失了产生或维持完整BL的能力，而其细胞在与血管外膜接触时保留了产生和重组沿着线性BL的能力。不能维持一个完整的BL可能涉及肿瘤破坏正常组织结构，以及在发展过程中的浸润性癌细胞的边界破裂。
A transplantable carcinoma of the rat pancreas that is composed of cytologically differentiated acinar cells that have lost their epithelial orientation and do not form acini was studied. Light microscopy shows consistent palisading, reorientation and polarization of these cells in areas of contact with the vasculature. EM reveals a normal basal lamina (BL) along the basal portions of repolarized tumor cells that is physically separate from the endothelial BL. Indirect immunofluorescence was used to examine the distribution of BL constituents, laminin (LM) and type IV collagen (type IV) within the different microenvironments of this tumor. In normal pancreas, Lm and type IV are distributed linearly, outlining acini and blood vessels. In the tumor parenchyma type IV is not detected, whereas Lm appears in a punctate distribution outlining cells. Reorientation of tumor cells is observed only along linearly deposited Lm and type IV bordering vessels. This nonmetastatic tumor has apparently lost the ability to produce or maintain a complete BL within its disorganized parenchyma, while its cells retain the capacity to produce and reorganize along linear BL when in contact with vascular adventitia. Failure to maintain a complete BL may be involved in the neoplastic disorganization of normal tissue architecture as well as in the breakdown of boundaries during the development of invasive carcinomata.