Transport proteins in bacteria: common themes in their design.
Transport proteins in bacteria: common themes in their design.
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细菌中的转运蛋白：其设计中的常见主题。
DOI：
10.1126/science.1279804
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
SaierJr,MH
中科院分区：
文献类型：
--
作者：
Nikaido,H;SaierJr,MH
细菌转运蛋白介导小溶质跨膜的被动和主动转运。氨基酸序列的比较显示，不仅在细菌转运蛋白之间，而且在它们与许多动物细胞的转运蛋白之间具有很强的保守性;因此，对细菌转运蛋白的研究有望有助于我们了解更复杂细胞中的转运蛋白。在过去的几年中，三种细菌外膜转运蛋白的结构被X射线晶体学解析。细菌细胞质膜转运蛋白的生物化学和分子遗传学研究也取得了很大进展，膜转运蛋白的统一设计正在逐步形成。具有不同能量耦合机制的转运体之间的共同结构动机和进化起源表明，许多转运体包含形成溶质可以通过的跨膜通道的中央模块。能量耦合机制可以被视为附加到这些基本易位单元上的次要特征。
Bacterial transport proteins mediate passive and active transport of small solutes across membranes. Comparison of amino acid sequences shows strong conservation not only among bacterial transporters, but also between them and many transporters of animal cells; thus the study of bacterial transporters is expected to contribute to our understanding of transporters in more complex cells. During the last few years, structures of three bacterial outer membrane transporters were solved by x-ray crystallography. Much progress has also occurred in the biochemical and molecular genetic studies of transporters in the cytoplasmic membranes of bacteria, and a unifying design among membrane transporters is gradually emerging. Common structural motives and evolutionary origins among transporters with diverse energy-coupling mechanisms suggest that many transporters contain a central module forming a transmembrane channel through which the solute may pass. Energy-coupling mechanisms can be viewed as secondary features added on to these fundamental translocation units.