ANALYSIS OF GASTRULATION - DIFFERENT TYPES OF GASTRULATION MOVEMENT ARE INDUCED BY DIFFERENT MESODERM-INDUCING FACTORS IN XENOPUS-LAEVIS
ANALYSIS OF GASTRULATION - DIFFERENT TYPES OF GASTRULATION MOVEMENT ARE INDUCED BY DIFFERENT MESODERM-INDUCING FACTORS IN XENOPUS-LAEVIS
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DOI：
10.1016/0925-4773(93)90021-o
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发表时间：
1993-09-01
影响因子：
2.6
通讯作者：
SMITH, JC
中科院分区：
文献类型：
--
作者：
HOWARD, JE;SMITH, JC
本文分析了非洲爪蟾原肠胚形成的控制。我们的方法利用了中胚层诱导因子如激活素、FGF和BMP-4诱导假定的外胚层细胞进行原肠胚样运动的观察结果。例如，激活素使完整的动物极区进行会聚延伸，并使单个细胞在纤连蛋白包被的基底上扩散和迁移。通过改变动物极细胞暴露的生长因子的浓度，并通过将它们应用于不同的组合，我们展示了如何分级分布的因素的组合可以建立正确的空间和时间模式的原肠胚在非洲爪蟾胚胎。我们提出的分布支持并发展了先前由绿色等人（1992）提出的模型，以解释早期胚胎中基因激活的空间模式。
In this paper we analyze the control of gastrulation in Xenopus laevis. Our approach takes advantage of the observation that mesoderm-inducing factors such as activin, FGF and BMP-4 induce presumptive ectodermal cells to undergo gastrulation-like movements. Activin, for example, makes intact animal pole regions undergo convergent extension and causes individual cells to spread and migrate on a fibronectin-coated substrate. By varying the concentrations of the growth factors to which animal pole cells are exposed, and by applying them in different combinations, we show how graded distributions of a combination of factors could establish the correct spatial and temporal patterns of gastrulation in the Xenopus embryo. The distributions we propose support and develop the model previously suggested by Green et al. (1992) to account for the spatial patterns of gene activation in the early embryo.