Antibiotic Manipulation of Intestinal Microbiota To Identify Microbes Associated with Campylobacter jejuni Exclusion in Poultry
Antibiotic Manipulation of Intestinal Microbiota To Identify Microbes Associated with Campylobacter jejuni Exclusion in Poultry
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DOI：
10.1128/aem.00678-10
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发表时间：
2010-12-01
影响因子：
4.4
通讯作者：
Weber, T. E.
中科院分区：
文献类型：
--
作者：
Scupham, A. J.;Jones, J. A.;Weber, T. E.
研究了家禽肠道微生物群的各种亚群保护火鸡免受空肠弯曲杆菌定殖的能力。通过用无弯曲杆菌的成年火鸡的盲肠内容物接种日龄家禽，然后用一种抗菌剂（维吉尼亚霉素、恩诺沙星、新霉素或万古霉素）治疗，在体内产生群落亚群。梭恩诺沙星-、新霉素-和万古霉素-衍生的群落的空肠负荷分别降低了1个对数、2个对数和4个对数。通过基于阵列的方法rRNA基因的寡核苷酸指纹图谱检测衍生群落的组成，检测到对C.空肠抑制治疗。
The ability of various subsets of poultry intestinal microbiota to protect turkeys from colonization by Campylobacter jejuni was investigated. Community subsets were generated in vivo by inoculation of day-old poults with the cecal contents of a Campylobacter-free adult turkey, followed by treatment with one antimicrobial, either virginiamycin, enrofloxacin, neomycin, or vancomycin. The C. jejuni loads of the enrofloxacin-, neomycin-, and vancomycin-derived communities were decreased by 1 log, 2 logs, and 4 logs, respectively. Examination of the constituents of the derived communities via the array-based method oligonucleotide fingerprinting of rRNA genes detected a subtype of Megamonas hypermegale specific to the C. jejuni-suppressive treatments.