Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Sclerotinia sclerotiorum
Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Sclerotinia sclerotiorum
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DOI：
10.1016/j.funbio.2012.04.015
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发表时间：
2012-07-01
期刊：
影响因子：
2.5
通讯作者：
Silva, Roberto Nascimento
中科院分区：
文献类型：
--
作者：
Cardoso Lopes, Fabyano Alvares;Steindorff, Andrei Stecca;Silva, Roberto Nascimento
木霉属的一些物种已成功地用于真菌病原体的商业生物防治，例如，核盘菌（Sclerotinia sclerotiorum）是菜豆（Phaseolus vulgaris L.）本研究的目的是（1）提供从巴西塞拉多分离的木霉菌株的分子特征;（2）通过Biolog FF微孔板评估每种菌株的代谢谱;（3）评估每种菌株拮抗S.通过产生细胞壁降解酶（CWDEs）、挥发性抗生素和双重培养试验，对油菜菌核病菌进行了研究。其中棘孢木霉占42.86%，哈茨木霉占33.33%，绒毛木霉占14.29%，拟康氏木霉占4.76%，刺猬木霉占4.76%.棘孢木霉表现出最高的CWDE活性。然而，没有物种分泌一组特定的CWDEs。棘孢木霉Trichoderma asperellum 364/01、T. asperellum 483/02和T. Asperellum 356/02对真菌寄生过程中的关键酶表现出较高和中等的比活性，但拮抗能力较低。我们观察到CWDE和拮抗作用之间，或代谢谱和拮抗作用之间没有显着的相关性。木霉属的物种多样性，特别是T。哈茨木属植物的代谢特征清楚地反映了这一点。我们的研究结果表明，选择木霉菌候选生物防治应主要基于竞争菌株和目标病原体的环境适应性。(C)2012年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
Some species of Trichoderma have successfully been used in the commercial biological control of fungal pathogens, e.g., Sclerotinia sclerotiorum, an economically important pathogen of common beans (Phaseolus vulgaris L.). The objectives of the present study were (1) to provide molecular characterization of Trichoderma strains isolated from the Brazilian Cerrado; (2) to assess the metabolic profile of each strain by means of Biolog FF Microplates; and (3) to evaluate the ability of each strain to antagonize S. sclerotiorum via the production of cell wall-degrading enzymes (CWDEs), volatile antibiotics, and dual-culture tests. Among 21 isolates, we identified 42.86 % as Trichoderma asperellum, 33.33 % as Trichoderma harzianum, 14.29 % as Trichoderma tomentosum, 4.76 % as Trichoderma koningiopsis, and 4.76 % as Trichoderma erinaceum. Trichoderma asperellum showed the highest CWDE activity. However, no species secreted a specific group of CWDEs. Trichoderma asperellum 364/01, T. asperellum 483/02, and T. asperellum 356/02 exhibited high and medium specific activities for key enzymes in the mycoparasitic process, but a low capacity for antagonism. We observed no significant correlation between CWDE and antagonism, or between metabolic profile and antagonism. The diversity of Trichoderma species, and in particular of T. harzianum, was clearly reflected in their metabolic profiles. Our findings indicate that the selection of Trichoderma candidates for biological control should be based primarily on the environmental fitness of competitive isolates and the target pathogen. (C) 2012 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.