Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros).
Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros).
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从马尔布鲁克（Chlorocebus cynosuros）中分离出猿猴免疫缺陷病毒。
DOI：
10.1007/s00705-016-3129-8
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Sawa H.
中科院分区：
文献类型：
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作者：
Carr M;Kawaguchi A;Sasaki M;Gonzalez G;Ito K;Thomas Y;Hang'ombe BM;Mweene AS;Zhao G;Wang D;Orba Y;Ishii A;Sawa H.
为了研究赞比亚非人灵长类动物（NHP）中猴免疫缺陷病毒（SIV）的多样性，对非洲绿色猴（AGM）外周血淋巴细胞与人CD 4 + T细胞系共培养回收的一种新型SIV进行了新一代测序，以确定其全基因组序列。我们报告了第一个描述的SIV（SIVagmMAL-ZMB）从malbrouck（Chlorocebus cynosuros）。通过脾RNA的实时PCR分析，在3.2%（3/94）的AGM中检测到SIVagmMAL-ZMB，在狒狒中检测不到（0/105）。SIVagmMAL-ZMB与已知的SIV分离株具有<80%的核苷酸序列同一性，并且在所有基因组中均位于黑长尾猴SIV株的基部。
To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.