Gene delivery by combination of novel liposomal bubbles with perfluoropropane and ultrasound
Gene delivery by combination of novel liposomal bubbles with perfluoropropane and ultrasound
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DOI：
10.1016/j.jconrel.2006.09.008
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发表时间：
2007-01-22
影响因子：
10.8
通讯作者：
Maruyama, Kazuo
中科院分区：
文献类型：
--
作者：
Suzuki, Ryo;Takizawa, Tomoko;Maruyama, Kazuo
微泡和超声波最近已被研究，以期提高非病毒基因递送系统的转染效率。然而，微泡是不稳定的，并且其靶向能力不足以用于临床使用。为了解决这些问题，我们开发了新型聚乙二醇（PEG）修饰的脂质体（气泡脂质体），含有全氟丙烷，这是一种超声成像气体。在这里，我们使用超声波在Bubble脂质体中诱导空化，然后研究它们在体外和体内递送基因的能力。泡状脂质体可将质粒DNA递送到多种细胞类型中，且无细胞毒性。此外，在体内基因传递中，气泡脂质体比脂质转染法更有效地传递到股动脉。因此，气泡脂质体可能是基因递送的有效且新型的非病毒工具。(c)2006 Elsevier B.V保留所有权利。
Microbubbles and ultrasound have recently been investigated with a view to improving the transfection efficiency of non-viral gene delivery systems. However, microbubbles are unstable and their targeting ability is insufficient for clinical use. To circumvent these problems, we developed novel polyethyleneglycol (PEG) modified liposomes (Bubble liposomes) containing perfluoropropane, which is an ultrasound imaging gas. Here, we used ultrasound to induce cavitation in Bubble liposomes and then investigated their ability to deliver genes in vitro and in vivo. Bubble liposomes could deliver plasmid DNA to many cell types without cytotoxicity. Additionally, in vivo gene delivery, Bubble liposomes were more effective delivery into femoral artery than lipofection method. Thus, Bubble liposomes might be efficient and novel non-viral tools for gene delivery. (c) 2006 Elsevier B.V All rights reserved.