Protein contact printing for a surface plasmon resonance biosensor with on-chip referencing
Protein contact printing for a surface plasmon resonance biosensor with on-chip referencing
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DOI：
10.1016/s0925-4005(00)00716-4
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发表时间：
2001-04-15
影响因子：
8.4
通讯作者：
Ratner, BD
中科院分区：
文献类型：
--
作者：
Lu, HB;Homola, J;Ratner, BD
蛋白质接触印刷（pCP）被用于局部功能化一种具有片上参考的新型波长调制单流道表面等离子体共振（SPR）传感器。SPR传感器具有高折射率的介质五氧化二钽（Ta2O5）覆盖部分金传感表面，产生SPR共振（SPR- ta），该共振与无Ta2O5的Au共振（SPR-Au）共振在光谱上分离。因此，提供了一个片上参考通道[Electron]。文献35(1999)1105]。该通信表明，pCP可用于功能化这种双通道SPR传感器。通过使用pCP，牛血清白蛋白（BSA）被证明可以很好地钝化表面，以防止非特异性蛋白质吸附。2,4-二硝基苯基化BSA （DNP-BSA）可被溶液中的抗二硝基苯基抗体（α -DNP）特异性识别。通过在Au和Ta2O5表面分别印刷DNPBSA和BSA，我们证明了这两种信号的可逆体折射率变化可以被抵消。因此，通过从SPR-Au信号中减去适当比例的SPR-Ta参考信号，可以得到更精确的α -DNP/DNP相互作用结合曲线。我们的研究表明，pCP是一种简单、高效和通用的方法，可以将具有足够表面覆盖率和活性的多种蛋白质递送到这些传感器表面，而不会对相邻区域产生交叉干扰。(C) 2001 Elsevier Science B.V.版权所有
Protein contact printing (pCP) has been applied to locally functionalize a novel wavelength-modulated single flow channel surface plasmon resonance (SPR) sensor with an on-chip reference. The SPR sensor has a high refractive index dielectric tantalum pentoxide (Ta2O5) overlayer covering part of the gold sensing surface to generate a SPR resonance (SPR-Ta) that is spectrally-separated from the resonance of the Ta2O5-free Au resonance (SPR-Au). Thus, an on-chip reference channel is provided [Electron. Lett. 35 (1999) 1105]. This communication demonstrates that pCP can be used for functionalizing such dual-channel SPR sensors. By using pCP bovine serum albumin (BSA) was shown to passivate the surfaces well enough to prevent non-specific protein adsorption. In contrast, the printed 2,4-dinitrophenylated BSA (DNP-BSA) was recognized specifically by anti-dinitrophenyl antibodies (alpha -DNP) in solution. By printing DNPBSA and BSA onto the Au and Ta2O5 surfaces, respectively, we demonstrate that the reversible bulk refractive index changes presented in both signals can be canceled out. Therefore, a more accurate binding curve for the alpha -DNP/DNP interaction can be obtained by subtracting the properly scaled SPR-Ta reference signal from the SPR-Au signal. We show that pCP is a simple, efficient, and versatile method for delivering multiple proteins with sufficient surface coverage and activity onto such sensor surfaces without cross-interference to adjacent areas. (C) 2001 Elsevier Science B.V. All rights reserved.