Genome-wide microRNA profiles identify miR-378 as a serum biomarker for early detection of gastric cancer
Genome-wide microRNA profiles identify miR-378 as a serum biomarker for early detection of gastric cancer
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DOI：
10.1016/j.canlet.2011.10.034
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发表时间：
2012-03-28
期刊：
影响因子：
9.7
通讯作者：
Xiao, Huasheng
中科院分区：
文献类型：
--
作者：
Liu, Hanshao;Zhu, Lin;Xiao, Huasheng
最近的研究表明，在几种人类恶性肿瘤中，循环microRNAs（miRNAs）的异常表达谱预示着巨大的癌症诊断潜力。在这里，我们表明血清miR-378可以作为胃癌（GC）检测的一种新的非侵入性生物标志物。实时定量RT-PCR（qRT-PCR）分析的全基因组miRNA表达谱显示，miR-187*、miR-371- 5 p和miR-378在GC患者中显著升高。进一步的验证表明，单独的miR-378可以产生ROC曲线面积为0.861，区分GC患者和健康对照的灵敏度为87.5%，特异性为70.73%。总的来说，这些数据支持我们的论点，即血清miR-378作为胃癌检测的新型非侵入性生物标志物具有很强的潜力。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Recent studies demonstrated that in several human malignancies aberrant expression profiles of circulating microRNAs (miRNAs) anticipate great cancer diagnostic potential. Here we showed that serum miR-378 could serve as a novel noninvasive biomarker in gastric cancer (GC) detection. Genome-wide miRNA expression profiles followed with Real-Time quantitative RT-PCR (qRT-PCR) assays revealed that miR-187*, miR-371-5p and miR-378 were significantly elevated in GC patients. Further validation indicated that miR-378 alone could yields a ROC curve area of 0.861 with 87.5% sensitivity and 70.73% specificity in discriminating GC patients from healthy controls. Collectively, these data support our contention that serum miR-378 has strong potential as a novel noninvasive biomarker in gastric cancer detection. (C) 2011 Elsevier Ireland Ltd. All rights reserved.