Quercetin-3-O-glucoside suppresses pancreatic cancer cell migration induced by tumor-deteriorated growth factors in vitro
Quercetin-3-O-glucoside suppresses pancreatic cancer cell migration induced by tumor-deteriorated growth factors in vitro
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DOI：
10.3892/or.2016.4598
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发表时间：
2016-04-01
期刊：
影响因子：
4.2
通讯作者：
Kim, Jae Hoon
中科院分区：
文献类型：
--
作者：
Lee, Jungwhoi;Lee, Jungsul;Kim, Jae Hoon
使用通用表达编码（UPC）与公共微阵列数据库GEO的分析表明，胰腺癌中VEGF-A、bFGF和bFGFR 2的mRNA表达显著高于正常胰腺组织中的mRNA表达。人胰腺癌细胞系CFPAC-1和SNU-213对外源性VEGF-A、bFGF和TGF-β 1的迁移敏感性存在相对差异。槲皮素-3-O-葡萄糖苷处理CFPAC-1细胞，即使在相对较低的剂量下，也能抑制TGF-β和VEGF-A诱导的迁移活性，但在bFGF激活的SNU-213细胞中则不然。然而，高剂量的槲皮素-3-O-葡萄糖苷足以抑制bFGF诱导的SNU-213细胞的迁移活性。此外，低剂量吉西他滨和槲皮素-3-O-葡萄糖苷联合处理对bFGF诱导的CFPAC-1和SNU-213细胞浸润活性具有协同抑制作用。这些结果共同表明，槲皮素-3-O葡萄糖苷可以作为胰腺癌中由各种生长因子诱导的局部转移的抑制剂，并且是目前用于胰腺癌的吉西他滨的化疗功效的有效佐剂。
Analysis using Universal exPress Codes (UPCs) with the public microarray database GEO indicates significantly higher mRNA expressions of VEGF-A, bFGF, and bFGFR2 in pancreatic cancers than those in normal pancreatic tissues. Human pancreatic cancer cell line CFPAC-1 and SNU-213 had relatively differential sensitivity to exogenous VEGF-A, bFGF, and TGF-beta 1 in migration property. Treatment of quercetin-3-O-glucoside suppressed the migratory activity induced by TGF-(3 and VEGF-A even at relatively low dosages in CFPAC-1, but not in bFGF-activated SNU-213 cells. However, high dosages of quercetin-3-O-glucoside sufficiently suppressed the migratory activity induced by bFGF in SNU-213 cells. Furthermore, co-treatment with low dose of gemcitabine plus quercetin-3-O-glucoside showed synergistic inhibition effects on the infiltrate activity induced by bFGF in CFPAC-1 and SNU-213 cells. These results collectively suggested that quercetin-3-O glucoside could act as an inhibitor of local metastasis induced by various growth factors in pancreatic cancers and be an effective adjuvant to boost chemotherapeutic efficacy of gemcitabine, currently used in pancreatic cancers.