Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse.
Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse.
复制标题
编码小鼠神经细胞粘附分子 (N-CAM) 的基因的染色体位置。
DOI：
10.1073/pnas.82.22.7631
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发表时间：
1985
影响因子：
11.1
通讯作者：
Cunningham,BA
中科院分区：
文献类型：
--
作者：
D'Eustachio,P;Owens,GC;Edelman,GM;Cunningham,BA
编码神经细胞粘附分子的基因N-CAM已经定位在小鼠9号染色体上。利用鸡N-CAM基因编码区对应的cDNA探针pEC204，筛选了以lambda噬菌体EMBL4为载体制备的BALB/cJ小鼠基因组文库。分离到4个弱反应性重组噬菌体和1个强反应性重组噬菌体。后者的一个与pEC204强烈同源的区域被亚克隆得到一个新的探针，pEC501。RNA转移印迹和核苷酸测序表明，pEC501编码部分小鼠N-CAM基因。该探针确定了一个独特的遗传位点Ncam，与限制性片段长度多态性相关，允许定义两个等位基因。通过将体细胞杂交细胞系中与探针反应的小鼠特异性DNA片段的存在或缺失与各种小鼠染色体的存在或缺失相关联，该位点可以暂时分配到9号染色体或10号染色体上。对小鼠重组自交系Ncam相关DNA多态性的遗传分析表明，Ncam与9号染色体上的Lap-1、Sep-1和Thy-1位点有密切的连锁关系。这一结果表明Ncam与小脑突变交错（sg）位点之间存在额外的联系。Ncam位点为绘制其他细胞粘附分子的基因以及参与神经发育和功能的其他分子的基因提供了重要的参考点。
The gene encoding the neural cell adhesion molecule, N-CAM, has been localized on mouse chromosome 9. A BALB/cJ mouse genomic library prepared in lambda bacteriophage EMBL4 was screened by using a cDNA probe, pEC204, that corresponds to the coding region of the chicken N-CAM gene. Four weakly reactive and one strongly reactive recombinant phage were isolated. A region of the latter that was strongly homologous to pEC204 was subcloned to yield a new probe, pEC501. RNA transfer blots and nucleotide sequencing indicated that pEC501 encoded part of the mouse N-CAM gene. This probe defined a unique genetic locus, Ncam, associated with a restriction fragment length polymorphism that allowed the definition of two alleles. The locus could be provisionally assigned either to chromosome 9 or to chromosome 10 by correlating the presence or absence of mouse-specific DNA fragments reactive with the probe in a panel of somatic hybrid cell lines with the presence or absence of the various mouse chromosomes. Analysis of the inheritance of the Ncam-associated DNA polymorphism in recombinant inbred strains of mice revealed close linkage between Ncam and the Lap-1, Sep-1, and Thy-1 loci on chromosome 9. This result suggests an additional linkage between Ncam and the locus for the cerebellar mutation staggerer (sg). The Ncam locus provides an important reference point for mapping the genes for additional cell adhesion molecules as well as genes for other molecules involved in neural development and function.