Inertial microfluidics for continuous particle separation in spiral microchannels
Inertial microfluidics for continuous particle separation in spiral microchannels
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DOI：
10.1039/b908271a
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Papautsky, Ian
中科院分区：
文献类型：
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作者：
Kuntaegowdanahalli, Sathyakumar S.;Bhagat, Ali Asgar S.;Papautsky, Ian
在这项工作中，我们报告了一个简单的惯性微流控装置，实现连续的多粒子分离使用的原则，迪恩耦合惯性迁移的螺旋微通道。由于曲线微通道几何形状，与Dean旋转力耦合的主导惯性力导致颗粒占据微通道内壁附近的单个平衡位置。粒子平衡的位置取决于惯性升力与迪恩阻力之比。利用这一概念，我们首次展示了一种螺旋芯片实验室（LOC），用于将多颗粒混合物中的颗粒根据尺寸聚焦在微通道横截面上的不同平衡位置。单个颗粒流可以用适当设计的出口系统收集。为了证明这一原理，使用具有500 μ m的固定宽度和130 μ m的高度的5环阿基米德螺旋微通道来同时和连续地分离10 μ m、15 μ m和20 μ m的聚苯乙烯颗粒。该装置表现出90%的分离效率。通过以80%的效率和高的相对活力（> 90%）分离神经母细胞瘤和胶质瘤细胞证明了该装置的多功能性。所实现的类似于1百万个细胞/分钟的通量显著高于由其它微尺度分选方法报道的分选速率，并且与用商业大尺度流式细胞术技术获得的速率相当。这种被动微流体方法提供的简单的平面结构和高通量使其在生物医学和环境应用中的微流控器件具有吸引力。
In this work we report on a simple inertial microfluidic device that achieves continuous multi-particle separation using the principle of Dean-coupled inertial migration in spiral microchannels. The dominant inertial forces coupled with the Dean rotational force due to the curvilinear microchannel geometry cause particles to occupy a single equilibrium position near the inner microchannel wall. The position at which particles equilibrate is dependent on the ratio of the inertial lift to Dean drag forces. Using this concept, we demonstrate, for the first time, a spiral lab-on-a-chip (LOC) for size-dependant focusing of particles at distinct equilibrium positions across the microchannel cross-section from a multi-particle mixture. The individual particle streams can be collected with an appropriately designed outlet system. To demonstrate this principle, a 5-loop Archimedean spiral microchannel with a fixed width of 500 mu m and a height of 130 mu m was used to simultaneously and continuously separate 10 mu m, 15 mu m, and 20 mu m polystyrene particles. The device exhibited 90% separation efficiency. The versatility of the device was demonstrated by separating neuroblastoma and glioma cells with 80% efficiency and high relative viability (> 90%). The achieved throughput of similar to 1 million cells/min is substantially higher than the sorting rates reported by other microscale sorting methods and is comparable to the rates obtained with commercial macroscale flow cytomerty techniques. The simple planar structure and high throughput offered by this passive microfluidic approach make it attractive for LOC devices in biomedical and environmental applications.