AMINO-ACIDS RESPONSIBLE FOR FLAGELLAR SHAPE ARE DISTRIBUTED IN TERMINAL REGIONS OF FLAGELLIN
AMINO-ACIDS RESPONSIBLE FOR FLAGELLAR SHAPE ARE DISTRIBUTED IN TERMINAL REGIONS OF FLAGELLIN
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DOI：
10.1016/0022-2836(91)90187-b
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发表时间：
1991-06-05
影响因子：
5.6
通讯作者：
YAMAGUCHI, S
中科院分区：
文献类型：
--
作者：
KANTO, S;OKINO, H;YAMAGUCHI, S
鼠伤寒沙门氏菌鞭毛的形状在通常情况下是左旋螺旋。除了正常的野生型丝状体外，在突变体或体外重组的丝状体中还发现了非螺旋（即直的）、右旋螺旋（卷曲的）或圆形（半卷曲和卷曲的）丝状体以及具有小振幅的丝状体（f1型）。我们分析了野生型鞭毛蛋白和鞭毛蛋白从17鞭毛形状的突变体（6直丝，6与卷曲丝，4与卷曲丝和1与F1型丝）通过氨基酸测序，以确定突变位点。所有的突变体鞭毛蛋白，除了f1型丝有单突变，f1型鞭毛蛋白有两个突变的分子。这些突变的位点位于鞭毛蛋白末端区域的α-螺旋片段。本文还讨论了鞭毛丝多态性的可能机制。
The shape of the flagellar filaments of the bacteriumSalmonella typhimurium, under ordinary conditions is a left-handed helix. In addition to the normal wild-type filament, non-helical (i.e.straight), right-handed helical (curly), or circular (semi-coiled and coiled) filaments and filament with small amplitude (f1-type) have been found in mutants or in filaments reconstitutedin vitro. We analysed wild-type flagellin and flagellins from 17 flagellar-shape mutants (6 with straight filaments, 6 with curly filaments, 4 with coiled filaments and 1 with f1-type filament) by amino acid sequencing to identify the mutational sites. All mutant flagellins except that of the f1-type filament had single mutations; the f1-type flagellin had two mutations in the molecule. The sites of these mutations were localized in α-helical segments of the terminal regions of flagellin. A possible mechanism of the polymorphism of the flagellar filament is discussed.