Effects of thymidine analogues on murine and human cells.
Effects of thymidine analogues on murine and human cells.
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胸苷类似物对小鼠和人类细胞的影响。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
P. Parsons
中科院分区：
文献类型：
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作者：
S. G. Smellie;P. Parsons
三种小鼠肿瘤细胞系在 3 µM 5-溴脱氧尿苷 (BUdR) 中连续生长。其中一个品系 (MC-2) 产生逆转录病毒，并在 BUdR 或 5-碘脱氧尿苷 (IUdR) 存在的情况下改变形态。这些效应可以通过在正常培养基中生长来逆转，与 B-16 小鼠黑色素瘤系的报道相似。然而，本研究中使用的 B-16 系以及多种人类细胞（六种黑色素瘤系和三种成纤维细胞株）对 BUdR 更加敏感，0.03-0.1 µM 是连续生长的最大耐受水平。 BUdR、脱氧尿苷 (UdR)、5-氟脱氧尿苷 (FUdR) 或胸苷 (TdR) 不会在这些细胞中诱导病毒产生或形态学变化。细胞标记和生长研究的结果表明，BUdR 掺入 DNA 与毒性存在相关性。在与 1 microM TdR 竞争的基础上进行比较，两种人类细胞系掺入 1 microM 核苷的顺序为 TdR = BUdR = IUdR 大于 UdR 大于 FUdR。与之前的报道相比，FUdR 掺入 RNA 但不掺入 DNA，一半的 FUdR 标记是在碱稳定、DNase 敏感的材料中发现的。超过 90% 的其他化合物被掺入 DNA 中。所有 UdR 和 60% 的 IUdR 标记均以胸苷形式掺入；在 FUdR 存在的情况下，可以通过标记来抑制这种转化。
Three mouse tumour cell lines grew continuously in 3 micro M 5-bromodeoxyuridine (BUdR). One line (MC-2) produced a retrovirus and altered in morphology in the presence of BUdR or 5-iododeoxyuridine (IUdR). These effects, which could be reversed by growth in normal medium were similar to those reported for the B-16 mouse melanoma line. The B-16 line used in this study, however, as well as a variety of human cells (six melanoma lines and three fibroblast strains), were much more sensitive to BUdR, 0.03-0.1 micro M being the maximum tolerated levels for continuous growth. No virus production or changes in morphology were induced in these cells by BUdR, deoxyuridine (UdR), 5-fluorodeoxyuridine (FUdR) or thymidine (TdR). The results of cell labelling and growth studies showed a correlation of incorporation of BUdR into DNA with toxicity. Compared on a competitive basis with 1 micro M TdR, the order of incorporation of 1 micro M nucleosides by two human cell lines was TdR = BUdR = IUdR greater than UdR greater than FUdR. In contrast to previous reports that FUdR is incorporated into RNA but not into DNA, half of the FUdR label was found in alkalistable, DNase-sensitive material. Over 90% of the other compounds was incorporated into DNA. All of the UdR and 60% of the IUdR label was incorporated as thymidine; this conversion could be inhibited by labelling in the presence of FUdR.