Identification and characterization of an apoptosis-stimulating protein of p53 (ASPP) gene from Branchiostoma belcheri: Insights into evolution of ASPP gene family
Identification and characterization of an apoptosis-stimulating protein of p53 (ASPP) gene from Branchiostoma belcheri: Insights into evolution of ASPP gene family
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文昌鱼 p53 凋亡刺激蛋白 (ASPP) 基因的鉴定和表征：深入了解 ASPP 基因家族的进化
DOI：
10.1016/j.fsi.2015.12.040
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发表时间：
2016
影响因子：
4.7
通讯作者：
Ma Fei
中科院分区：
文献类型：
--
作者：
Song Xiaojun;Du Juan;Zhu Wei;Jin Ping;Ma Fei
在P53依赖和非P53非依赖途径中，ASPP蛋白家族在细胞凋亡调控中起着非常关键的作用。然而，到目前为止，在文昌鱼中还没有发现ASPP同源基因。在这里，我们从Branchiostoma belcheri(设计为AmphiASPP)中克隆并鉴定了一个ASPP基因，并对其进化及其在先天性免疫中的作用进行了广泛的研究。结果表明，文昌鱼基因组中存在一个ASPP同源基因，其开放阅读框架为3285bp，编码1094个氨基酸，含有ANK重复序列和SK3结构域。进化分析表明，ASPP蛋白家族成员可能存在于媒介线虫的共同祖先中，并在进化史上经历了正向选择。此外，文昌鱼ASPP基因在所研究的五个组织中普遍存在并存在差异表达，文昌鱼ASPP基因参与了内毒素和LTA刺激的先天免疫反应。生物信息学分析表明，文昌鱼ASPP蛋白与.REL蛋白通过保守的结合部位相互作用，与人ASPP2蛋白相比，文昌鱼ASPP蛋白可能通过NF-кB信号通路参与天然免疫。综上所述，我们的发现为文昌鱼ASPP家族的进化和天然免疫功能提供了新的见解。
The ASPP (apoptosis-stimulating protein of p53) protein family plays very key roles in apoptosis regulation, in both p53-dependent and p53-independent pathways. However, the ASPP homologous gene has.not been identified in amphioxus to date. Here, we identified and characterized an ASPP gene from.Branchiostoma belcheri (designed as AmphiASPP) and extensively studied its evolution and roles involved.in innate immunity. The results showed that the amphioxus genome has an ASPP homolog gene with an.ORF of 3285 bp, encoding 1094 amino acids which contains ANK repeats and SK3 domain. The evolutionary analyses indicated that the members of ASPP protein family might be present in a common.ancestor of Nematostella vectensis and underwent positive selective in the evolutionary history. In.addition, the amphioxus ASPP gene was ubiquitously and differentially expressed in five investigated.tissues, and the amphioxus ASPP gene was involved in the innate immune response of LPS and LTA.stimulation. Finally, bioinformatic analyses displayed that amphioxus ASPP protein could interact with.REL protein by conserved binding sites compared with human ASPP2 protein, which seemed to further.suggest that the amphioxus ASPP protein involve in innate immunity through NF-кB signaling pathway..Taken together, our findings provided an insight into the evolution and innate immunity function of the.ASPP family.