Determination of Serum BRAK/CXCL14 Levels in Healthy Volunteers
Determination of Serum BRAK/CXCL14 Levels in Healthy Volunteers
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DOI：
10.1309/lmqoxcqef7zxiiuk
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发表时间：
2010-08-01
期刊：
影响因子：
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通讯作者：
Hata, Ryu-Ichiro
中科院分区：
文献类型：
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作者：
Izukuri, Kazuhito;Ito, Shin;Hata, Ryu-Ichiro
背景：Brak/CXCL14是一种在许多正常细胞和组织中表达的趋化因子。已有多篇文献报道了该趋化因子的组织分布，但尚无研究对该趋化因子的血清蛋白水平进行检测。方法：在确定了提高方法灵敏度和重复性所需的条件后，我们用酶联免疫吸附试验测定了血清中Brak/CXCL14的水平。结果：男性的平均水平显著高于女性，2%的受试者BRAK/CXCL14的平均值高于平均值，无明显异常。结论：血清BRAK/CXCL14水平高度保守，提示BRAK/CXCL14水平是由个体遗传背景和/或生活方式决定的。
Background: BRAK/CXCL14 is a chemokine expressed in many normal cells and tissues. Several papers have reported tissue distribution, but no study has examined the serum protein level of this chemokine.Methods: We evaluated serum levels of BRAK/CXCL14 by enzyme-linked immunosorbent assay, after determining the conditions necessary to increase the sensitivity and reproducibility of the methodResults: The average level of males was significantly higher than that of females Two percent of the volunteers showed a value 2 S.D. higher than the average value without any obvious abnormality, but no person was lower than 1 S D of the average value The serum BRAK/CXCL14 levels of individual subjects were constantConclusion: These data indicate that the serum BRAK/CXCL14 level is highly conserved and suggest that the levels are determined by the genetic background and/or lifestyle of the individual.