Cell culture of sixteen‐day‐old rat embryo cerebra and associated changes in ganglioside pattern
Cell culture of sixteen‐day‐old rat embryo cerebra and associated changes in ganglioside pattern
复制标题
十六日龄大鼠胚胎大脑的细胞培养及神经节苷脂模式的相关变化
DOI：
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发表时间：
1977
影响因子：
4.7
通讯作者：
M. A. Repman
中科院分区：
文献类型：
--
作者：
C. Schengrund;M. A. Repman
THE GANGLIOSIDE composition of neurons (essentially neuronal perikarya) and glia prepared using bulk isolation techniques has been reported to be similar to that obtained for whole brain (ABE & NORTON, 1974; HAMBERGER & SVENNERHOLM, 1971). In contrast, mouse neuroblastoma cells (often used as a possible model for neurons) and glioma cells grown in uitro have simplified ganglioside patterns. The neuroblastoma cells lack trisialoand sometimes disialogangliosides (SCHENGRUND & NELSON, 19766; STOOLMILLER et a!., 1973) and the glioma cells have only hematoside (STOOLMILLER et al., 1973). Bulk preparations of neuronal perikarya, and neuroblastoma and glioma cells grown in uitro, all have low levels of ganglioside-sialidase (EC 3.2.1.18 neuraminidase, N-acetylneuraminosyl glycohydrolase) activity relative to that of mature brain (SCHENGRUND & NELSON, 1976a, 6). This study was done to determine whether nontransformed brain cells dissociated from rat embryo cerebra and grown in uitro displayed the ganglioside pattern characteristic of these cells in the CNS, and an increasing sialidase activity, as they differentiate morphologically in uitro.
