Microneedle-Based Potentiometric Sensing System for Continuous Monitoring of Multiple Electrolytes in Skin Interstitial Fluids
Microneedle-Based Potentiometric Sensing System for Continuous Monitoring of Multiple Electrolytes in Skin Interstitial Fluids
复制标题
DOI：
10.1021/acssensors.0c02330
复制
发表时间：
2021-05-26
期刊：
影响因子：
8.9
通讯作者：
Zhang, Yi
中科院分区：
文献类型：
--
作者：
Li, Huijie;Wu, Guangfu;Zhang, Yi
电解质在调节心血管功能、水合作用和肌肉激活方面起着关键作用。目前用于监测电解质的标准涉及定期的血液采样和使用实验室技术的测量，这通常对受试者来说是不舒服/不方便的，并且增加了对其潜在疾病状况的管理的相当大的费用。皮肤间质液（ISF）中广泛的电解质及其与血浆中电解质的相关性为电解质和昼夜代谢监测等应用创造了令人兴奋的机会。然而，监测皮肤ISF中的这些电解质具有挑战性。在这项研究中，我们报告了一种微创微针电位传感系统的多路复用和连续监测Na（+）和K+在皮肤ISFs。电位传感系统由一个小型化的不锈钢空心微针，以防止传感器分层和一组修改后的微针电极的多重监测。我们证明了测量Na+和K+在人工ISFs中具有快速的响应时间，优异的可逆性和重复性，足够的选择性，以及添加生理相关浓度的代谢物，饮食生物标志物和营养素时可忽略的潜在干扰。此外，传感器在多次插入鸡皮模型后仍保持灵敏度。此外，使用校准的传感器在人工ISF中的测量证实了人工ISF中生理电解质的准确测量。最后，皮肤模拟体模凝胶和鸡皮肤模型实验证明了传感器的潜在微创监测皮肤ISF中的电解质。所开发的传感器平台可以适用于广泛的其他应用，包括营养物、代谢物和蛋白质的真实的实时监测。
Electrolytes play a pivotal role in regulating cardiovascular functions, hydration, and muscle activation. The current standards for monitoring electrolytes involve periodic sampling of blood and measurements using laboratory techniques, which are often uncomfortable/inconvenient to the subjects and add considerable expense to the management of their underlying disease conditions. The wide range of electrolytes in skin interstitial fluids (ISFs) and their correlations with those in plasma create exciting opportunities for applications such as electrolyte and circadian metabolism monitoring. However, it has been challenging to monitor these electrolytes in the skin ISFs. In this study, we report a minimally invasive microneedle-based potentiometric sensing system for multiplexed and continuous monitoring of Na(+)and K+ in the skin ISFs. The potentiometric sensing system consists of a miniaturized stainless-steel hollow microneedle to prevent sensor delamination and a set of modified microneedle electrodes for multiplex monitoring. We demonstrate the measurement of Na+ and K+ in artificial ISFs with a fast response time, excellent reversibility and repeatability, adequate selectivity, and negligible potential interferences upon the addition of a physiologically relevant concentration of metabolites, dietary biomarkers, and nutrients. In addition, the sensor maintains the sensitivity after multiple insertions into the chicken skin model. Furthermore, the measurements in artificial ISFs using calibrated sensors confirm the accurate measurements of physiological electrolytes in artificial ISFs. Finally, the skin-mimicking phantom gel and chicken skin model experiments demonstrate the sensor's potential for minimally invasive monitoring of electrolytes in skin ISFs. The developed sensor platform can be adapted for a wide range of other applications, including real time monitoring of nutrients, metabolites, and proteins.