Genetic analysis of cob resistance to F. verticillioides: another step towards the protection of maize from ear rot
Genetic analysis of cob resistance to F. verticillioides: another step towards the protection of maize from ear rot
复制标题
玉米穗轴对轮叶镰刀菌抗性的遗传分析：保护玉米免受穗腐病的又一步
DOI：
10.1007/s00122-018-3258-4
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发表时间：
2019-04-01
影响因子：
5.4
通讯作者：
Wu, Jianyu
中科院分区：
文献类型：
--
作者：
Mu, Cong;Gao, Jingyang;Wu, Jianyu
通过鉴定候选抗病基因，为进一步研究玉米对轮枝镰刀菌穗轴病的抗性奠定了基础。轮枝镰刀菌穗腐病是黄淮海平原最常见的玉米穗腐病类型。抗穗腐病包括抗穗轴病和抗籽粒病。目前的文献大多集中在籽粒抗性上，而对穗轴抗性的遗传研究很少。本研究的目的是鉴定影响F.轮枝病抗性。利用全基因组关联研究，鉴定出与48个单核苷酸多态性（SNP）相关的28个基因（P < 10-4）与FCR抗性相关。FCR抗性的主要数量性状位点qRcfv 2通过连锁作图在2号染色体上被鉴定，并在近等基因系群体中被验证。在qRcfv 2区域检测到2个与2个SNPs相关的候选基因，阈值较低（P < 10-3）。通过实时荧光定量PCR检测，发现其中一个候选基因在玉米穗轴中不表达，而另一个候选基因在F.轮枝状这些结果为深入研究玉米棒的抗病机理奠定了基础，也为分子标记辅助选择提供了资源。
We lay the foundation for further research on maize resistance to Fusarium verticillioides cob rot by identifying a candidate resistance gene. Fusarium verticillioides ear rot is the most common type of maize ear rot in the Huanghuaihai Plain of China. Ear rot resistance includes cob and kernel resistance. Most of the current literature concentrates on kernel resistance, and genetic studies on cob resistance are scarce. We aimed on identifying the QTLs responsible for F. verticillioides cob rot (FCR) resistance. Twenty-eight genes associated with 48 single nucleotide polymorphisms (SNPs) were identified (P < 10-4) to correlate with FCR resistance using a whole-genome association study. The major quantitative trait locus, qRcfv2, for FCR resistance was identified on chromosome 2 through linkage mapping and was validated in near-isogenic line populations. Two candidate genes associated with two SNPs were detected in the qRcfv2 region with a lower threshold (P < 10-3). Through real-time fluorescence quantitative PCR, one candidate gene was found to have no expression in the cob but the other was expressed in response to F. verticillioides. These results lay a foundation for research on the resistance mechanisms of cob and provide resources for marker-assisted selection.