Extreme spore UV resistance of Bacillus pumilus isolates obtained from an ultraclean spacecraft assembly facility
Extreme spore UV resistance of Bacillus pumilus isolates obtained from an ultraclean spacecraft assembly facility
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DOI：
10.1007/s00248-003-1029-4
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发表时间：
2004-02-01
影响因子：
3.6
通讯作者：
Nicholson, W
中科院分区：
文献类型：
--
作者：
Link, L;Sawyer, J;Nicholson, W
最近对美国宇航局喷气推进实验室的超清洁航天器组装设施进行的环境微生物采样确定，短小芽孢杆菌的孢子是在航天器及其周围发现的主要可培养细菌污染物。作为评估各种航天器灭菌剂效果的努力的一部分，10个JPL-SAF短小芽孢杆菌分离株的纯化孢子受到254 nm紫外线照射，并将它们的紫外线抗性与标准枯草杆菌孢子的生物剂量学菌株进行了比较。在10个JPL-SAF菌株中，有6个菌株的孢子对紫外线的抗性显著高于枯草芽孢杆菌，其中JPL-SAF菌株之一的短小芽孢杆菌SAFR-032的抗紫外线能力是芽孢杆菌中最高的。到目前为止遇到的。
Recent environmental microbial sampling of the ultra-clean Spacecraft Assembly Facility at NASA Jet Propulsion Laboratory (JPL-SAF) identified spores of Bacillus pumilus as major culturable bacterial contaminants found on and around spacecraft. As part of an effort to assess the efficacy of various spacecraft sterilants, purified spores of 10 JPL-SAF B. pumilus isolates were subjected to 254-nm UV and their UV resistance was compared to spores of standard B. subtilis biodosimetry strains. Spores of six of the 10 JPL-SAF isolates were significantly more resistant to UV than the B. subtilis biodosimetry strain, and one of the JPL-SAF isolates, B. pumilus SAFR-032, exhibited the highest degree of spore UV resistance observed by any Bacillus spp. encountered to date.