PARP-14 binds specific DNA sequences to promote Th2 cell gene expression.
PARP-14 binds specific DNA sequences to promote Th2 cell gene expression.
复制标题
DOI：
10.1371/journal.pone.0083127
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Goenka S
中科院分区：
文献类型：
--
作者：
Riley JP;Kulkarni A;Mehrotra P;Koh B;Perumal NB;Kaplan MH;Goenka S
PARP-14, a member of the poly ADP-ribose polymerase super family, promotes T helper cell 2 (Th2) differentiation by regulating interleukin-4 (IL-4) and STAT6-dependent transcription. Yet, whether PARP-14 globally impacts gene regulation has not been determined. In this report, using an RNA pol II ChIP-seq approach, we identify genes in Th2 cells that are regulated by PARP-14, and either dependent or independent of ADP-ribosyltransferase catalytic activity. Our data demonstrate that PARP-14 enhances the expression of Th2 genes as it represses the expression of Th1-associated genes. Among the relevant targets are Signal Transducer and Activator of Transcription genes required for polarizing Th1 and Th2 cells. To define a mechanism for PARP-14 function, we use an informatics approach to identify putative PARP-14 DNA binding sites. Two putative PARP-14 binding motifs are identified in multiple Th2 cytokine genes, and we demonstrate that PARP-14 interacts with each motif using in vitro binding assays. Taken together our results indicate that PARP-14 is an important factor for T helper cell differentiation and it binds to specific DNA sequences to mediate its function.
登录
查看更多内容
影响因子：
4
作者：
Gao, PS;Heller, NM;Huang, SK
通讯作者：
Huang, SK
影响因子：
4.8
作者：
Goenka, Shreevrat;Cho, Sung Hoon;Boothby, Mark
通讯作者：
Boothby, Mark
影响因子：
30.5
作者：
Avni, O;Lee, D;Rao, A
通讯作者：
Rao, A
影响因子：
16
作者：
Heinz S;Benner C;Spann N;Bertolino E;Lin YC;Laslo P;Cheng JX;Murre C;Singh H;Glass CK
通讯作者：
Glass CK
影响因子：
32.4
作者：
Kaplan, MH;Schindler, U;Grusby, MJ
通讯作者：
Grusby, MJ