Dynamic and Progressive Control of DNA Origami Conformation by Modulating DNA Helicity with Chemical Adducts
Dynamic and Progressive Control of DNA Origami Conformation by Modulating DNA Helicity with Chemical Adducts
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DOI：
10.1021/acsnano.6b01339
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发表时间：
2016-05-01
期刊：
影响因子：
17.1
通讯作者：
Choi, Jong Hyun
中科院分区：
文献类型：
--
作者：
Chen, Haorong;Zhang, Hanyu;Choi, Jong Hyun
DNA折纸因其能够以几纳米的精度对复杂的纳米结构进行编程而受到了极大的关注。动态折纸结构可以根据环境线索或外部信号改变构象，在纳米级的传感和驱动方面前景广阔。现有动态折纸结构的重构机制大多局限于单链铰链，几乎完全依赖于DNA杂交或链位移。在这里，我们展示了另一种方法，通过展示DNA结合分子的按需构象变化，这些分子插入碱基对之间并解开DNA双螺旋。解绕效应调节了DNA折纸过程中的螺旋错配，对折纸结构的内应力和整体构象有显著影响。我们展示了聚合折纸纳米带在不同扭转状态之间的切换以及单体折纸轴中良好约束的扭转变形。结构转变是可逆的，结合等温线证实了重构机制。该方法提供了一种快速可逆的方法来改变DNA折纸构象，可用于纳米尺度上的动态和渐进控制。
DNA origami has received enormous attention for its ability to program complex nanostructures with a few nanometer precision. Dynamic origami structures that change conformation in response to environmental cues or external signals hold great promises in sensing and actuation at the nanoscale. The reconfiguration mechanism of existing dynamic origami structures is mostly limited to single-stranded hinges and relies almost exclusively on DNA hybridization or strand displacement. Here, we show an alternative approach by demonstrating on-demand conformation changes with DNA-binding molecules, which intercalate between base pairs and unwind DNA double helices. The unwinding effect modulates the helicity mismatch in DNA origami, which significantly influences the internal stress and the global conformation of the origami structure. We demonstrate the switching of a polymerized origami nanoribbon between different twisting states and a well-constrained torsional deformation in a monomeric origami shaft. The structural transformation is shown to be reversible, and binding isotherms confirm the reconfiguration mechanism. This approach provides a rapid and reversible means to change DNA origami conformation, which can be used for dynamic and progressive control at the nanoscale.