Induction of Ucp2 expression in brain phagocytes and neurons following murine toxoplasmosis:: An essential role of IFN-γ and an association with negative energy balance
Induction of Ucp2 expression in brain phagocytes and neurons following murine toxoplasmosis:: An essential role of IFN-γ and an association with negative energy balance
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DOI：
10.1016/j.jneuroim.2007.03.013
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发表时间：
2007-05-01
影响因子：
3.3
通讯作者：
Richard, Denis
中科院分区：
文献类型：
--
作者：
Arsenijevic, Denis;Clavel, Sebastien;Richard, Denis
利用小鼠弓形虫病模型研究解偶联蛋白-2 （Ucp2）在大脑中的细胞和时间表达。原位杂交表明Ucp2位于神经元中。参与能量平衡的核结构，特别是孤立束核（NST），被证明在负能量平衡和Ucp2水平之间有正相关。感染诱导的Ucp2表达主要与小胶质细胞共定位，但也与脑内浸润的巨噬细胞和中性粒细胞共定位，这在感染后第9天就很明显了。通过细胞因子敲除小鼠，我们证明了大脑中小胶质Ucp2的诱导在很大程度上依赖于干扰素- γ，而不是白细胞介素-6或肿瘤坏死因子- α。总之，本研究表明，感染后，Ucp2在神经元中的调节方式与在小胶质细胞/吞噬细胞中的调节方式不同。我们的研究表明，负能量平衡与NST中神经元Ucp2水平之间存在关联。(c) 2007 Elsevier B.V.版权所有
A model of murine toxoplasmosis was used to study cellular and temporal expression of uncoupling protein-2 (Ucp2) in the brain. In situ hybridization indicated that Ucp2 was located in neurons. Nuclei structures involved in energy balance, in particular the nucleus of the solitary tract (NST), was shown to have a positive association between negative energy balance and Ucp2 levels. Infection-induced Ucp2 expression colocalized mainly with microglial cells, but also with infiltrating macrophages and neutrophils in the brain, which was evident from day 9 post-infection. Using cytokine knockout mice we demonstrate that microglial Ucp2 induction in the brain was largely dependant on interferon-gamma, but not interleukin-6 or tumour-necrosis-factor-alpha in response to infection. In summary, this study shows that Ucp2 is regulated in a different manner in neurons than in microglia/phagocytes following infection. Our study indicates that an association exists between negative energy balance and neuronal Ucp2 levels in the NST, in particular. (c) 2007 Elsevier B.V. All rights reserved.