Lycopene production from synthetic medium by Blakeslea trispora NRRL 2895 (+) and 2896 (-) in a stirred-tank fermenter
Lycopene production from synthetic medium by Blakeslea trispora NRRL 2895 (+) and 2896 (-) in a stirred-tank fermenter
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Blakeslea trispora NRRL 2895 ( ) 和 2896 (-) 在搅拌罐发酵罐中从合成培养基生产番茄红素
DOI：
10.1007/s00449-011-0654-4
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发表时间：
2012-06-01
影响因子：
3.8
通讯作者：
Tang, Ya-Jie
中科院分区：
文献类型：
--
作者：
Liu, Xiu-Ji;Liu, Rui-Sang;Tang, Ya-Jie
20%空气饱和度的溶解氧张力、第3天pH从4.0转变到5.5以及适度的剪切应力（按叶轮叶尖速度计算，V - 叶尖 = 0.926 - 2.161米/秒）被确定为将三孢布拉氏霉菌NRRL 2895（+）和2896（ - ）用于生产番茄红素的交配发酵从摇瓶放大到搅拌罐发酵罐的关键因素。在7.5升搅拌罐发酵罐中获得了最高183.3毫克/升的番茄红素产量，然后交配发酵过程成功地从7.5升逐步放大到200升搅拌罐发酵罐。发酵过程的可比性得到了很好的控制，并且在放大过程中番茄红素产量得以维持。此外，在第3天综合添加150微摩尔/升脱落酸，在第4天添加0.5克/升亮氨酸和0.1克/升青霉素，在搅拌罐发酵罐中三孢布拉氏霉菌的交配发酵中实现了最高270.3毫克/升的番茄红素产量。
The dissolved oxygen tension of 20% of air saturation, pH-shift from 4.0 to 5.5 on day 3, and a moderate shear stress (calculated as an impeller tip speed, V-tip - 0.926 - 2.161 m/s) were identified to be the key factors in scaling-up the mated fermentation of Blakeslea trispora NRRL 2895 (+) and 2896 (-) for lycopene production from a shake flask to a stirred-tank fermenter. The maximal lycopene production of 183.3 mg/L was obtained in 7.5-L stirred-tank fermenter, and then the mated fermentation process was successfully step-wise scaled-up from 7.5- to 200-L stirred-tank fermenter. The comparability of the fermentation process was well controlled and the lycopene production was maintained during the process scale-up. Furthermore, with the integrated addition of 150 mu mol/L abscisic acid on day 3, 0.5 g/L leucine and 0.1 g/L penicillin on day 4, the highest lycopene production of 270.3 mg/L was achieved in the mated fermentation of B. trispora in stirred-tank fermenter.