Fifty sequenced-tagged sites on human chromosome 11.
Fifty sequenced-tagged sites on human chromosome 11.
复制标题
人类 11 号染色体上有 50 个测序标记位点。
作者：
T. Miwa;K. Sudo;Y. Nakamura;T. Imai
从定位于人类11号染色体的粘粒克隆中鉴定了50个新的序列标记位点（STS）。通过与体细胞杂交板杂交，确定了69个粘粒标记的一个或两个克隆末端的DNA序列，这些标记各自定位于24个亚染色体区域中的1个。确定每个标记物的适当引物序列和聚合酶链反应（PCR）的适当条件。其中21个克隆不适合产生STS，主要是因为它们的两端都含有Alu和L1序列等重复元件;然而，有些克隆不适合产生STS，因为它们作为模板的人DNA的PCR产物的大小与酵母DNA的相同。最后，从48个克隆中建立了50个STS，其中20个来自短臂标记，30个来自长臂标记。这些STS可作为研究体细胞杂种中的人类DNA和分离酵母人工染色体的新试剂，以锚大的DNA重叠群和11号染色体的精细物理图谱。
Fifty novel sequenced-tagged sites (STSs) were identified from cosmid clones mapped to human chromosome 11. DNA sequences were determined for one or both cloning ends of 69 cosmid markers that had each been localized to 1 of 24 subchromosomal regions by means of hybridization to somatic cell hybrid panels. Proper primer sequences and appropriate conditions for a polymerase chain reaction (PCR) were determined for each marker. Twenty-one of the cosmids were not suitable for generating STSs, mainly because both of their ends contained repetitive elements such as Alu and L1 sequences; however, some were inappropriate because the sizes of their PCR products from human DNA, used as template, were same as those from yeast DNA. Finally, 50 STSs were established from 48 clones: 20 were derived from markers localized on the short arm and 30 from the long arm. These STSs can serve as new reagents for investigating human DNA in somatic cell hybrids and for isolating yeast artificial chromosomes to anchor large DNA contigs and fine-scale physical maps of chromosome 11.