Transformation and plasmacytoid differentiation of EBV-infected human B lymphoblasts by ras oncogenes.
Transformation and plasmacytoid differentiation of EBV-infected human B lymphoblasts by ras oncogenes.
复制标题
ras 癌基因对 EBV 感染的人 B 淋巴母细胞的转化和类浆细胞分化。
DOI：
10.1126/science.2536954
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Dalla-Favera,R
中科院分区：
文献类型：
--
作者：
Seremetis,S;Inghirami,G;Ferrero,D;Newcomb,EW;Knowles,DM;Dotto,GP;Dalla-Favera,R
用嗜酸性逆转录病毒载体将H-或N-ras癌基因导入EB病毒永生化的人B淋巴母细胞，研究ras癌基因激活B细胞的生物学效应。H-和N-ras癌基因的表达导致这些细胞的恶性转化，如在半固体培养基中的克隆形成性和在免疫缺陷小鼠中的致瘤性所示。此外，终末分化为浆细胞可检测到形态学、免疫球蛋白分泌和细胞表面抗原表达的特异性变化。这种促进人类淋巴母细胞生长和分化的联合作用，代表了癌基因的一种新的生物学作用，并对终末分化的B淋巴恶性肿瘤如多发性骨髓瘤的发病机制有影响。
The biological effects ofrasoncogene activation in B cells were studied by using amphotropic retroviral vectors to introduce H- or N-rasoncogenes into human B lymphoblasts immortalized by Epstein-Barr virus. Expression of both H- and N-rasoncogenes led to malignant transformation of these cells, as shown by clonogenicity in semisolid media and tumorigenicity in immunodeficient mice. In addition, terminal differentiation into plasma cells was detectable as specific changes in morphology, immunoglobulin secretion, and cell surface antigen expression. This combined effect, promoting growth and differentiation in human lymphoblasts, represents a novel biological action ofrasoncogenes and has implications for the pathogenesis of terminally differentiated B-lymphoid malignancies such as multiple myeloma.