Purification of Sarcophaga (fleshfly) lectin and detection of sarcotoxins in the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina.
Purification of Sarcophaga (fleshfly) lectin and detection of sarcotoxins in the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina.
复制标题
麻蝇（肉蝇）凝集素的纯化和 NIH-Sape-4（麻蝇胚胎细胞系）培养基中肉毒素的检测。
DOI：
10.1042/bj2480217
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
S. Natori
中科院分区：
文献类型：
--
作者：
H. Komano;E. Kasama;Y. Nagasawa;Y. Nakanishi;K. Matsuyama;K. Ando;S. Natori
人们发现源自 Sarcophaga peregrina（肉蝇）胚胎的已建立细胞系 NIH-Sape-4 可以合成 Sarcophaga 凝集素和肌毒素 IA 的 mRNA，但不能合成储存蛋白或 25 kDa 蛋白的 mRNA。已知这四种蛋白质在三龄幼虫的脂肪体中合成，尤其是前两种蛋白质已知参与该昆虫的防御机制，并响应体壁损伤而被诱导。因此，胚胎细胞系NIH-Sape-4组成型合成某些防御蛋白。该细胞系可用于大规模纯化Sarcophaga凝集素，因为可以从约400ml培养基中获得50微克纯化的Sarcophaga凝集素。
An established cell line originating from a Sarcophaga peregrina (fleshfly) embryo, NIH-Sape-4, was found to synthesize mRNAs for Sarcophaga lectin and sarcotoxin IA, but not those for storage protein or 25 kDa protein. These four proteins are known to be synthesized in the fat-body of third-instar larvae, and the two former in particular are known to participate in the defence mechanism of this insect and to be induced in response to injury of the body wall. Thus the embryonic cell line NIH-Sape-4 synthesizes certain defence proteins constitutively. This cell line will be useful for large-scale purification of Sarcophaga lectin, since 50 micrograms of purified Sarcophaga lectin could be obtained from about 400 ml of culture medium.