Extraction and Analysis of Fumonisins and Compounds Indicative of Fumonisin Exposure in Plant and Mammalian Tissues and Cultured Cells
Extraction and Analysis of Fumonisins and Compounds Indicative of Fumonisin Exposure in Plant and Mammalian Tissues and Cultured Cells
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DOI：
10.1007/978-1-61779-102-4_15
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Riley, Ronald T.
中科院分区：
文献类型：
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作者：
Zitomer, Nicholas C.;Riley, Ronald T.
伏马菌素真菌毒素是许多谷物中常见的污染物，通常含量很低。玉米是特别成问题的，因为通常产生伏马菌素的生物体之一，真菌轮枝镰刀菌（Fusarium verticillioides）通常作为玉米的内生菌存在。伏马菌素是酶神经酰胺合酶的有效抑制剂，并且这种抑制导致多种上游化合物的积累，最值得注意的是鞘氨醇、鞘氨醇、1-脱氧鞘氨醇以及植物中的植物鞘氨醇。伏马菌素暴露导致多种物种、性别和菌株特异性反应。该方法提供了从组织和细胞中提取伏马菌素、鞘氨醇碱和鞘氨醇碱1-磷酸盐的相对快速的手段，以及使用液相色谱/串联质谱法对这些化合物进行随后的分析和定量。
Fumonisin mycotoxins are common contaminants in many grains, often at very low levels. Maize is particularly problematic as one of the organisms that commonly produce fumonisins, the fungus Fusarium verticillioides, often exists as an endophyte of maize. Fumonisin is a potent inhibitor of the enzyme ceramide synthase, and this inhibition results in the accumulation of a variety of upstream compounds, most notably, the sphingoid bases sphingosine, sphinganine, 1-deoxysphinganine and, in plants, phytosphingosine. Fumonisin exposure results in a wide variety of species, sex, and strain-specific responses. This method provides a relatively fast means of extracting fumonisins, sphingoid bases, and sphingoid base 1-phosphates from tissues and cells, as well as the subsequent analyses and quantification of these compounds using liquid chromatography/tandem mass spectrometry.