The combined extraction of sage (Salvia officinalis L.): Ultrasound followed by supercritical CO2 extraction
The combined extraction of sage (Salvia officinalis L.): Ultrasound followed by supercritical CO2 extraction
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DOI：
10.1016/j.ultsonch.2010.06.011
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发表时间：
2011-01-01
影响因子：
8.4
通讯作者：
Skala, Dejan U.
中科院分区：
文献类型：
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作者：
Glisic, Sandra B.;Ristic, Mihajlo;Skala, Dejan U.
采用超临界流体萃取（SFE）、水蒸气蒸馏法分离鼠尾草挥发性成分（单萜和倍半萜）、索氏提取法和超声波辅助提取法分离高分子化合物等方法，可以从鼠尾草中分离出多种植物化学成分。超声波辅助提取，然后用超临界CO2再提取所获得的提取物的组合进行了这项研究。进行调查的目的是集中鼠尾草提取物中存在的二萜，这通常被认为是负责提取化合物的抗氧化活性的分馏使用超临界CO2，和不同的组合的超声辅助溶剂提取（水-乙醇混合物或只有水），然后超临界CO2再提取所获得的提取物或处理的植物材料进行了分析和比较。根据这些调查的结果，它可以提出最佳的提取程序：超声波预处理的植物材料与蒸馏水和植物材料（残渣），使用超临界CO2的再提取。该方法得到两种有价值的产品：富含糖并具有免疫调节活性的超声提取物和富含二萜和倍半萜的超临界提取物。(C)2010 Elsevier B. V.保留所有权利。
A wide spectrum of phytochemicals could be isolated from sage (Salvia officinalis L) using different extraction or distillation technique: the supercritical fluid extraction (SFE), the volatiles compounds (monoterpenes and sesquiterpenes) isolation using hydrodistillation or higher molecular compounds with Soxhlet extraction or ultrasound-assisted extraction. The combination of ultrasound-assisted extraction followed by re-extraction of obtained extract with supercritical CO2 was performed in this study. The goal of performed investigation was to concentrate diterpenes present in sage extract which are generally considered to be responsible for antioxidant activity of extracted compounds.The fractionation using the supercritical CO2, and different combination of the ultrasound-assisted solvent extractions (water-ethanol mixture or only water) followed by supercritical CO2 re-extraction of obtained extract or treated plant material were analyzed and compared. Based on the results of these investigations it could be proposed the best extraction procedure: the ultrasound pretreatment of plant material with distilled water and re-extraction of plant material (residue) using supercritical CO2. That procedure gives two valuable products: the ultrasound extract which is rich in sugars and possess the immunomodulatory activity and supercritical extract which is rich in diterpenes and sesquiterpenes. (C) 2010 Elsevier B.V. All rights reserved.