Kinetics of adenovirus DNA replication II. Initiation of adenovirus DNA replication.
Kinetics of adenovirus DNA replication II. Initiation of adenovirus DNA replication.
复制标题
腺病毒 DNA 复制动力学 II。
DOI：
10.1016/0042-6822(80)90037-9
复制
发表时间：
1980
期刊：
影响因子：
3.7
通讯作者：
Pearson,GD
中科院分区：
文献类型：
--
作者：
Bodnar,JW;Pearson,GD
我们已经开发了三种方法来估计腺病毒DNA复制的起始：(a)在密度转移实验期间的直接测量，(b)使用病毒DNA积累的饱和动力学计算，以及(c)使用病毒DNA复制的稳态动力学计算。每种方法测量的起始速率的值在定量上一致，并证实了起始是腺病毒复制的限速步骤的假设。新复制的病毒DNA分子被优先启动。这种偏好在感染早期是轻微的，但在感染过程中明显增加。我们将这些结果解释为腺病毒被划分为几个池，并且至少一个池包含用于复制的分子。该池的平均起始率为0.0042±0.0007起始率/min/end，在整个感染过程中保持恒定。复制池的最大容量为50,000±7000分子/细胞。稳态计算表明，位移综合比互补综合速度快。这些合成速率之间的差异导致I/II型复制分子中新生链的过早位移，我们称之为过早位移合成。我们认为这一过程为产生缺陷腺病毒DNA分子提供了原料。
We have developed three methods to estimate the initiation of adenovirus DNA replication: (a) direct measurement during density-shift experiments, (b) calculation using the saturation kinetics of viral DNA accumulation, and (c) calculation using the steady-state kinetics of viral DNA replication. The values for the rate of initiation measured by each method agree quantitatively and confirm the postulate that initiation is the rate-limiting step in adenovirus replication. Newly replicated viral DNA molecules are preferentially initiated. This preference is slight early in infection, but increases markedly over the course of infection. We interpret these results to mean that adenovirus is partitioned into several pools, and at least one pool contains molecules destined for replication. The average rate of initiation in this pool is 0.0042 ± 0.0007 initiation/min/end and is constant throughout infection. The maximum size of the replication pool is 50,000 ± 7000 molecules/cell. Steady-state calculations indicate that displacement synthesis is faster than complementary synthesis. The differential between these rates of synthesis leads to premature displacement of nascent strands in type I/II replicating molecules, a process we call premature displacement synthesis. We propose that this process provides the raw material for generating defective adenovirus DNA molecules.