Drought tolerance in citrus rootstocks is associated with better antioxidant defense mechanism
Drought tolerance in citrus rootstocks is associated with better antioxidant defense mechanism
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DOI：
10.1007/s11738-018-2710-z
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发表时间：
2018-08-01
影响因子：
2.6
通讯作者：
Anjum, Muhammad Akbar
中科院分区：
文献类型：
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作者：
Hussain, Sajjad;Khalid, Muhammad Fasih;Anjum, Muhammad Akbar
柑橘是世界范围内种植的主要水果作物之一。柑橘树受到不同非生物胁迫的影响，包括干旱，导致其产量下降。在本研究中，对六种不同的柑橘砧木（Volkameriana 柠檬、巴西酸橙、Carrizo 柑橘、尤里卡柠檬、Gada dahi 和 Rangpur 酸橙）进行干旱胁迫（24 天），并测量了叶片相对含水量 (LRWC)、叶绿素“a”和“b”、抗氧化能力 (AC)、总酚含量和脯氨酸含量 (PRO)。在砧木的叶和根中测量过氧化氢（H2O2）、脂质过氧化（MDA）、总可溶性蛋白（TSP）和酶抗氧化活性，例如超氧化物酶歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）。干旱胁迫下的 Volkameriana 柠檬的 LRWC 最低，但叶片中的 SOD 活性较高。卡里佐枳橙叶子中的叶绿素“a”和“b”含量最低，根中的 MDA 和 H2O2 含量较低，但叶子和根中的 TSP 含量较高。在干旱胁迫下，尤里卡柠檬和朗布尔酸橙叶片中的 SOD、CAT、POD、PRO、MDA、H2O2 和 AC 降低。巴西酸橙（一种干旱敏感砧木）叶子中的 MDA 和 H2O2 含量较高。然而，卡里佐枳橙（一种耐旱砧木）根部的 SOD 活性较高，叶子和根部的 CAT 和 POD 活性较高。结果表明，所有研究的砧木的防御机制都不同。耐性砧木表现出较少量的 MDA 和 H2O2 以及较高的抗氧化酶活性（SOD、CAT 和 POD），以应对干旱胁迫期间产生的活性氧。
Citrus is one of the major fruit crops grown worldwide. Citrus trees are affected by different abiotic stresses including drought which decrease its yield. In this study, six different citrus rootstocks (Volkameriana lemon, Brazilian sour orange, Carrizo citrange, Eureka lemon, Gada dahi, and Rangpur lime) were subjected to drought stress (24 days) and leaf relative water content (LRWC), chlorophyll 'a' and 'b', antioxidant capacity (AC), total phenolic content, and proline content (PRO) were measured. Hydrogen peroxide (H2O2), lipid peroxidation (MDA), total soluble proteins (TSP), and enzymatic antioxidant activities, such as superoxidase dismutase (SOD), catalase (CAT), and peroxidase (POD), were measured in leaves and roots of the rootstocks. Drought-stressed Volkameriana lemon had the minimum LRWC but higher SOD activity in its leaves. Carrizo citrange possessed the minimum amount of Chl 'a' and 'b' in leaves and exhibited lesser MDA and H2O2 in roots but greater TSP content in leaves and roots. SOD, CAT, POD, PRO, MDA, H2O2, and AC decreased in the leaves of Eureka lemon and Rangpur lime under drought stress. MDA and H2O2 contents were observed higher in leaves of Brazilian sour orange (a drought sensitive rootstock). However, higher SOD activity in roots and higher CAT and POD activities in leaves and roots were recorded in Carrizo citrange (a drought-tolerant rootstock). The results indicated that all the studied rootstocks were different in their defense mechanism. Tolerant rootstocks exhibited less amount of MDA and H2O2 and more antioxidant enzymatic activities (SOD, CAT, and POD) to cope with reactive oxygen species produced during drought stress.